. 8 - 
of 1:4 hydrochloric acid and 5 cc. of 12-percent silicotungstic acid and, 
after 12 hours, col].ect the precip-'.tate in a G-ooch cruciols and dry at 
120°. The T7eight of precipitate x 0.1001 = grams of anahasine. Ho'.vever , 
in order to avoid aixy admixt'ore v^hlch vro'oJ-d give a similar precipitate, 
acidify slightly the 100 cc. of distillate with acetic acid and add 50 cc . 
of sat-ijirated picric acid solution. After 12 hours filter through a G-ooch 
crucihle and dry to constant Tveight at 650. On acco^ont of the soluhility 
of the salt, the Tveight of the precipitate x 0.29 = grams of anatasine. 
finally, a third quantitative procedure is offered ty precipitation ^7ith 
picrolouic acid, as described "before, and drying of the salt at 70°. 
The v/eight x 0.234 gives very good results. By this proced'ire it is 
possible to obtain anahasine picrolonate even in concentrations v/hich no 
longer jrield any precipitates with nicotine. 
liiown solutions of p-orified anabasine were analyzed with these re- 
agents. The results ootained with the different reagents were a,s follows: 
(l) 12-percent silicotungstic acid solution, precipitate dried at 120° C 
(assuming precipitate of 12^703- Si022C3_QHi4N2.2E20) , loiind 0.0088 gram 
anahasine (in 100 cc), theoretical 0.0090 gram; (2) cold- saturated 
aqueous picric acid solution, precipitate dried at 65° C. (using 
th.eoretical factor 0.258), foiJind 0.0080 gram (in 150 cc), theoretical 
0.0090 gram; (3) cold- saturated aqueous picrolonic acid, precipitate 
dried at 70° C. (using theoretical factor 0.234), found 0.0292, 0.0143, 
0.0072, and 0.003 gram (in 200 cc. ) , theoretical 0.0287, 0.0143, 0.0072, 
and 0.003 gram. 
I^tz (52) in 1937 reported that anabasine sulfate and nicotine sul- 
fate have many reactions in common. Sokolw (141) has shown that 
iTj'-c'rofluosilicic acid is an excellent reagent for precipitating anaoasine 
fluosilicate in the presence of nicotine, and it is nov shown that if an 
ethereal solution of nicotine is mixed with an equal voTome of ethereal 
iodine solution a precipitate of Cxo2i4^'2-^2*^-^ is formed, whereas 
anahasine does not give a similar precipitate . 
Surkat (15) in 1937 described qualitative reactions of anahasine 
vdth picric acid, phosphcmolybdic acid, and mercuric chloride. For a 
quantitative determinant ion, the alkaloid is distilled from sodium 
liydroxide and sodium chloride into standard acid and hack titrated. 
Zerhey, Orinick, and '"illard (152) in 1937 reported comparative 
tests of anabasine and its related, compuunds, its purification, and some 
physical constants. Anabasine is a colorless or pa-le-yellow liquid when 
pure, miscible with water in all proportions, and, like nicotine, it 
darkens on standing. An attempt was made to prepare p-ure ana-basine from 
its sulfate by treating with 20-percent sodium hydroxide, allowing to 
stand overnight, and extracting the oil layer with ether. The oil thus 
obtained contained all the alkaloids. It was distilled under reduced 
press\ire and separated into 21 fractions. Determinations of the density 
by the micro-pj'cnometer method, of the optical rotation with 5-percent 
solutions in alcohol, and of the refractive index with an Abbe refrac- 
tometer were made on fractions 2, 5, 8, 11, 16, and 19. Since the 
values were different with each fraction tested, it was impossible to 
tell which one corresponded to pure anabasine, but it is probable that 
n20^ 1.536-1.538, E^O,, 1.046-1.048, and [alpha]^^= -76.2 to 79.1°. 
A -liable is given showing the behavior of anabasine and the related 
