- 2- 
Experiment 1 
In the first experiment five eases of tobacco were placed in cold 
storage at approximately -10° F. In three of the cases the tobacco was 
in medium order, in one case it was dry, and in one case was soft. The 
term "order" refers to a condition of the tobacco leaf in which it contains 
sufficient moisture to be pliable and handled readily without breaking. In 
these experiments the tobacco in medium order contained approximately 
15 to 16 percent of moisture, dry tobacco contained less moisture (usually 
about 14 percent), and soft tobacco more moisture (usually about 17 
percent). 
The capacity of the cold-storage chamber was approximately 3,000 
cubic feet, and the cold air was circulated in the chamber. Test lots of 
cigarette beetles v/ere placed in the center of each case. 
At the beginning of the experiment the temperature of the tobacco was 
72° F. Later the temperatures in the cold-storage room and of the 
tobacco at the center of a case were as follows: 
Cold-storage room Tobacco 
After 45 hours 
After 69 hours 
After 93 hours 
-10°F. 
- 8 
- 8 
26° F. 
8 
4 
Unfortunately, thermocouples were not available for this experiment. 
The temperature readings, obtained by means of a long metal-jacketed 
thermometer thrust into the tobacco, were thought to be unreliable, 
because in an air temperature of -10° F. it is possible that the cold 
would be carried into the bulb of the thermometer by the metal jacket. 
After 4 days the cases of tobacco were removed from cold storage 
and the insects were removed and examined. Mortalities of adults and 
larvae were recorded after 3 to 4 hours and incubation of eggs and 
emergence of adults after 3 to 6 days. Table 1 shows that only a few of 
the test insects survived, except in the soft tobacco. 
Table 1. --Percent survival of cigarette beetles in the center of cases of 
cigar tobacco exposed for 4 days to approximately -10° F. 
Type of 
tobacco 
Moisture con- 
dition of tobacco 
Adult 
Larvae 
Pupae 
Eggs 
Wisconsin binder 
Dry 




Medium 
order 

2 


Pennsylvania filler 
Soft 
41 
93 
40 
7 
Connecticut binder 
Medium 
order 
8 
18 
1 

Havana seed binder 
-do.- 




Pennsylvania filler 
(check) 
-do.- 
98 
100 
100 
96 
