-3 
Leaf-tobacco experts carefully examined all the tobacco several days 
after it had been removed from cold storage. They concluded that none 
of it had been adversely affected by exposure to cold. All the tobacco was 
dry and hard when first removed from the cold room, but as it warmed 
up it absorbed moisture from the air. After 2 to 4 days the tobacco had 
returned to normal summer temperature, and was at least in as good 
condition as before it was exposed. Some of the dry tobacco was slightly 
improved, as it had picked up a little more moisture. 
Experiment 2 
In the second experiment 10 cases of tobacco were placed in cold 
storage at approximately -10 F., as described in the first experiment. 
Four of the cases were of Pennsylvania filler, four of Wisconsin binder, 
and two of Connecticut binder. All the tobacco was in medium order 
except one case of Pennsylvania filler, which was soft. Five cases, 
including the one of soft tobacco, were held in cold storage for 3 days, 
one case was held for 4 days, one for 5, and three for 6 days. In two 
cases thermocouples were placed at 5, 10, 15, and 21 inches from the 
approximate center of one end. The thermocouple 21 inches from the 
end was at approximately the center of the mass of tobacco. One of 
these cases was the soft tobacco held in cold storage for 3 days, and the 
other case was in medium order and held for 6 days. 
Test lots of the various stages of the cigarette beetle were placed in 
each case- -4, 8, 12, and 16 inches deep into the tobacco from the approxi- 
mate center of one end. Check lots were placed at the same locations in 
four cases of the same types of tobacco, and held at summer storage 
temperature- -about 80° -85° F. The temperature of all the tobacco at 
the beginning of the experiment was 84-86° F. 
The decreases in temperature of the tobacco at different depths in 
the case are shown in figure 1. At a depth of 5 inches only 1 day was 
required for the tobacco temperature to fall to 0° F., as compared with 
more than 3 days at 10 inches and more than 5 days at 15 inches. At 
21 inches, in the center of the case, the temperature dropped a little 
more slowly than at 15 inches during the first 4 days, but by the sixth 
day the temperatures at these two locations were the same. 
Table 2 shows that a 3-day exposure killed all insects at the 4-inch 
depth, and also at the 8 -inch depth in the tobacco of medium order. As 
in the first experiment, an appreciable percentage of larvae and adults 
survived at this 8-inch depth in the soft tobacco. After 4 days' exposure 
mortality was complete at both the 12- and 16-inch depths in all tobacco. 
