4- 
Table 2. --Percent survival of cigarette beetles at different depths in 
cases of cigar tobacco held for 3 days in cold storage at approximately 
-10° F. 
Moisture condition 
of tobacco 
Depth of insects 
in tobacco 
Adults 
Larvae 
Pupae 
Eggs 
Medium order 
Soft 
Medium order (check) 
Inches 
4 




8 




12 

8 


16 

7 


4 




8 
56 
48 
4 

12 
100 
60 
36 
2 
16 
48 
80 
44 
5 
4 
100 
98 
100 
100 
8 
100 
100 
99 
98 
12 
100 
98 
95 
100 
16 
100 
98 
96 
98 
Experiment 3 
In the third experiment four cases of tobacco were placed in a com- 
mercial cold-storage plant at -20° F. for 3 days. The air in this plant 
was not circulated as in the other tests. One case was dry, two cases 
were in medium order, and one was soft. In three cases, thermocouples 
were placed 4, 8, 12, and 16 inches from the approximate center of one 
end. Test lots of all stages of the cigarette beetle were placed in each 
case, as in the preceding experiment. Check insects were held at room 
temperature (75°-80°). 
The temperature decrease was slightly greater in the dry tobaccc 
than in the tobacco of medium order, and slightly less in the soft 
tobaccc (fig. 2). 
The effect of the moisture content of the tobacco on the mortality of 
test insects is shown in table 3. All the insects were killed at the 4-inch 
depth. At the 8-inch depth there was no survival in dry tobacco, negli- 
gible survival in tobacco in medium order, and appreciable survival, 
especially of larvae, in soft tobacco. At the 12- and 16-inch depths 
survival increased with the moisture content of the tobacco. 
