96 
BULLETIN OF THE BUREAU OF FISHERIES 
for both the stomach and the intestine. This layer of granule cells is very prominent 
in the pyloric caeca of the king salmon. Normally the layer is from two to four cells 
deep between the outer surface of the stratum compactum and the circular muscle coat. 
These cells are supported by the delicate strands of the outer surface of the stratum 
compactum. A few granule cells are located on the inner wall of the stratum com- 
pactum and occasional ones are found in the tunica propria, as mentioned above. 
The numerous relatively large granule cells suggest Oppel's granule cells of Protem 
in structural characteristics. The granule cells of the king salmon, however, have 
not been found in the epithelial coat, though under pathological conditions they undoubt- 
edly do wander into other near-by regions. Gulland says, speaking of the pyloric 
appendages and the intestine: “ In both structures the eosinophile leucocytes are numer- 
ous, but are to be found mainly in the connective tissue about the stratum compactum.” 
In discussing these cells in connection with the structure of the stomach attention was 
called to the fact that Gulland figured cells of undoubtedly the same type as present in the 
king salmon. But I must again assert that these can not be blood leucocytes of the 
eosinophile or any other type. 
The granule cells of the king salmon cseca have small round nuclei placed more or 
less eccentric with reference to the center of the cell. The cell body itself varies extremely 
in outline. In the majority of instances this outline is that of a broad oval, but where 
a cell is located in a restricted mesh its shape is adapted to conform to the space. This 
is especially to be noted in those cells present in the tunica propria. 
In a section of a c*cum of a Monterey salmon taken June 23, 1911, the following 
measurements of granule ceils are noted: 8 by 12, 8 by 10, 5 by 13, 8 by 14, and 9 by 
14 /(. The special characteristic of the granule cells is the presence in the cytoplasm of 
large refractive granules. These granules stain with eosin, with Mallory’s stain, taking 
especially the Bismarck brown but occasionally the acid fuchsin. They stain sharply 
with iron haematoxylin. The granules are very evenly distributed throughout the 
cytoplasm. The cells outside the stratum compactum seem more uniformly crowded 
with granules by whatever method of staining they be studied, and more densely granular 
than in the granule cells of the stomach. Those scattered cells that are present in the 
tunica propria are the ones that present the greatest irregularity in the distribution of 
the granules. A greater irregularity in outline suggests that the cells are amoeboid. 
In one or sometimes both poles of these irregularly shaped cells the granules are fewer 
in number, though the difference is only slight. Also in the smaller cells there are on 
the average fewer granules than in the larger. The number of granule cells in the normal 
tissue is such that the connective tissue meshes seem about one-half filled with the cells. 
In the relatively distended ctecum the total thickness of the layer is less. These points 
are mentioned for comparison with the structural condition found in degenerated caeca 
in which the number and relations of the granule cells differ sharply from the normal 
Monterey salmon type, as will be described in a paper to follow. 
