CARCINOMA OP THE THYROID IN SARMONOID FISHES. 
461 
Tabi.e; VIII. — Feeding and Other Experiments with Wild Brook Trout. 
Tank. 
I^ot. 
.Fish. 
Food. 
Other conditions. 
Fish 
added 
Sept. 21, 
1910. 
Clinical condition, June, 1912.® 
Remain- 
ing. 
Clean. 
With red 
floors. 
With tu- 
mors. 
3 
4 
5 
6 
7 
8 
9 
10 
11 
12 
13 upper. 
13 lower. . 
14 
15 
16 
2146 
2147 
2148 
2149 
2150 
2151 
2152 
2153 
2154 
2155 
2156 
2159 
2160 
2161 
2162 
2163 
2164 
Heart, raw. . . 
Liver, raw. . . 
Marine fish... 
Liver, raw. . . 
Vegetable. . . . 
. . . .do 
Liver, cooked 
Liver, raw. . . 
Natural food. . 
Liver, raw. . . 
Natural food. 
... .do 
... .do 
do 
Reduced space; crowding. 
In presence of tumor fish. . 
.do. 
.do. 
.do. 
In presence of tumor fish 
Minimum feeding 
Forced feeding 
Controls 
Pond 10 mud in tank 
Controls for tanks 12-16 
Inoculated in thyroid region 
with trout thyroid tumor. 
Pond 10 mud in stomachs 
Inoculated in thyroid region 
with trout thyroid tumor. 
Inoculated behind eye with fil- 
trate from pond 10 mud. 
m' 
36 
Sec- 
tions, 
1911. 
Diagnoses, 1911. 
Sec- 
tions, 
1912. 
2000 
2001 
2002 
2098 
2099 
A, beginning hyperplasia; B, normal; C, carci- 
noma. 
2102 
2005 
2006 
2103 
2104 
2113 
2105 
2008 
2107 
2012 
2109 
2014 
2015 
2016 
2112 
Tank. 
Diagnoses, 1912 
3 
4 
5 
6 
7 
8 
9 
10 
11 
12 
13 Upper. 
13 lower . . 
14 
15 
16 
A, tumor, carcinoma; B, adeno-carcinoma. 
A, B, C, regression. 
Normal. 
Regression. 
Normal. 
Regression. 
A, B, regression. 
Normal. 
Regression. 
Normal. 
Increased thyroid of normal type. 
Increased thyroid of normal type. 
Normal. 
A, normal; B, increased thyroid of normal type. 
Increased thyroid of normal type. 
Increased thyroid of normal type. 
® Examinations of Nov. 30, 1910, and July, 1911, all were clean. 
& Transferred to pond 10. 
DESCRIPTION OF SECTIONS TO ACCOMPANY TABLE VIII. 
2000 B. Tubulo-alveolar type of carcinoma. Marked infiltration of surrounding areolar tissue. Infiltration of muscle above 
into bone spaces and infiltration of vessel wall. Invasion of cartilage. 
2001 A. Beginning hyperplastic stage. Certain follicles are lined with columnar epithelium. In many such follicles colloid is 
absent, in others present but poorly stained. Surrotmding this group of follicles are follicles of strictly normal appearance. Epi- 
thelium flattened, filled with colloid. Beginning of the hyperplasia in this specimen is evidently limited to individual follicles 
(Fig. 84.) 
2001 B. Most of the thyroid tissue in this specimen is of typical normal appearance, flattened epithelium, follicles filled with 
stainable colloid. 
2001 C. The entire space surrounding the vessel is filled with closely packed alveoli, small sized alveolar structures lined with 
high columnar epithelium, with deeply staining vesicular nuclei. Colloid is almost entirely absent. Evidently growth of the 
thyroid tissue which has spread upward into the areolar tissue immediately below the mucosa of the floor of the mouth. Under 
high power the nuclei present great variability in size, the staining qualities are vesicular with one or two nucleoli. Marked 
evidence of hyperemia between the follicles. Many tubules are filled with closely packed cells. In some portions of the 
growth all alveolar structure has disappeared and small islands of closely packed cells are found. Infiltration of dense con- 
nective tissue structure, perichondrium and periosteum. Beginning carcinoma of tubulo-alveolar type. 
60289° — Bull. 32 — 14 30 
