34 
MINIMUM LETHAL DOSE METHOD OF ASSAY. 
The method adopted for examination and the animals employed 
by us as illustrating the toxic methods of assay were as follows: 
Mice . — White mice, kept under the same conditions and varying in 
weight from 15 to 25 grams, were used. The drug was injected hypo- 
dermically beneath the skin of the back, the dose being calculated at 
so many milligrams per gram of body weight and so diluted that a 
dose of 1 c. c. was not exceeded in any case. The mice were then 
watched and the death or survival of the animal noted. The data 
thus obtained (as were the results in all subsequent series of experi- 
ments) were confirmed by injecting a second series. of mice several 
days later. The results of these tests are given in Table I. 
Table I . — Determination of the minimum lethal dose per gram body weight for white 
mice ; subcutaneous injection. 
MULFORD NO. 1. 
Number of animals used. a 
Dose. 
Results. 
1 
mgm. 
2 
4 
3 
5 
4 
+ 
3 
5 
+ 
3 
6 
+ 
MERRELL.& 
1 
3 
1 
4 
_ 
2 .. 
5 
_ 
1. 
5 
+ 
3 
6 
+ 
2 
7 
+ 
B. W. AND CO. 
3 
3 
2 
4 
+ 
1 
5 
+ 
1 
6 
+ 
2 
7 
+ 
H. B. AND W. 
3 
6 
1 
7 
_ 
3 
7 
+ 
2 
8 
+ 
1 ... 
9 
+ 
DIGITALONE NO. 1. 
1 
10 
1 
19 
_ 
1 
20 
_ 
1 
22 
+ 
1 
25 
+ 
1 
28 
+ 
1 
30 
+ 
a The heading “Number of animals used” refers 
indicated in the second column, 
fr Dose calculated on the basis that preparation wai 
LLOYD BROS. 
Number of animals used.® 
Dose. 
Results. 
2 
mgm. 
18 
2 
19 
2 
20 
— 
1 
20 
+ 
2 
21 

1 
21 
+ 
1 
22 
2 
22 
+ 
3 
23 
2 
23 
+ 
2 
24 
4 
24 
+ 
N. B. AND CO 
3 
7 
2 
8 
_ 
2 
8 
+ 
3 
9 
+ 
1 
11 
+ 
i. AND D. 
2 
6 
2 
7 
4 
8 
| r Sks 
1 
8 
+ 
2 
9 
3 
9 
+ 
1 
10 
3 
10 
+ 
P., D. AND CO. 
2 
3 
3 
4 
+ 
3 
4 
2 
5 
+ 
3 
5 
2 
6 
+ 
3 
6 
2 
7 
+ 
3 •_ 
7 
4 
8 
+ 
to the number of animals which received the dose 
of fluid extract strength. 
