36 
Table II. — Determination of the minimum lethal dose for guinea pigs — subcutaneous 
injection — Continued . 
DIGITALONE NO. 3. P., D. AND CO. 
Number of animals used. 
Dose. 
Result. Number of animals used. 
Dosd. 
Result. 
1 
mgm. 
0.90 
1.50 
- l. 
mgm. 
0. 50 
.60 
.70 
.70 
.80 
+ 
+ 
1 
a— 1... 
i 
H. B. AND TV. 
2 
2 
S. AND D. 
1 
0.30 
.40 
.50 
.70 
1.00 
i | 
- i 
0.70 
.80 
.80 
.90 
.90 
+ 
+ 
2 
— 3 
2 
+ 1. 
i 
+ 1. 
i 
+ 4... 
a No symptoms. 
Digitalone No. 3 was used in injecting two guinea pigs, one receiv- 
ing 0.9 milligram and the other 1.5 milligrams per gram weight. 
Neither of these animals showed any symptoms whatever except- 
ing that they were somewhat less active than normal. 
SUMMARY. 
Preparation. 
M. L. D.a 
mgm. 
B. W. and Co 0. 35 
H. B.andW .50 
Merrell .60 
Mulford No. 1 .70 
P., D. and Co 1 .70 
N., B. and Co .90 
S. and D .90 
Lloyd Bros .90 
Digitalone No. 1 5. 00(?) 
Digitalone No. 3 & 1. 50 
o M. L. D.=minimum lethal dose. 
& Lived. 
Cats . — The toxic dose was determined for these animals in the 
course of the blood-pressure experiments. After the initial injec- 
tion of 1 c. c. of the 10 per cent solution, further injections of 0.5 
c. c. each were administered at r intervals of not less than 5 minutes, 
artificial respiration being maintained so that the toxic action was 
due to an effect upon the heart rather than upon the medulla. This 
was thought desirable because in some of the earlier experiments the 
respiration was observed to have failed, although a good circulation 
was being maintained, while in others the animal would make appar- 
ently normal respiratory efforts after the heart had stopped and the 
blood pressure had fallen to zero. The toxic doses were compared 
on the basis of the number of milligrams of the drug used per kilo- 
gram of body weight of the animal. The results of this series of 
experiments and the average for each drug used are given in the 
last two columns of Table III. 
