46 
Table VI. — Determination of toxic dose for Dana pipiens, Focke method — drug injected 
into leg lymph sacs. 
Time is noted when circulation in web of foot stops. Dose is calculated per gram body weight, animals 
weighing between 25 and 30 grams each 
MULFORD NO. 1. N., B. AND CO. 
Dose. 
Time. 
Value (V). 
c. c. 
0.020 
Min. 
18 
j 
2.8 
.020 
18 
2.8 
.020 
14 
3.6 
.0225 
9 
4.9 
.0225 
12 
3.7 
.023 
11 
3.9 
.025 
9 
4.4 
.025 
9 
4.4 
Average 
V =3. 8 
MERRELL.o 
Dose. 
Time. 
Value (V). 
c. c. 
0.015 
Min. 
27 
.020 
18 
2.7 
.020 
17 
2.9 
.0225 
10 
4.4 
.025 
20 
2.0 
.026 
12 
3.2 
.027 
15 
2.5 
.030 
13i 
2.5 
.035.. 
10 
2.9 
Average 
j 
V=2.9 
H. B. AND W. 
0.035 
20 1.4 
.035 
15 2.0 0.0325 1 
14 
2.2 
.036 . 
16 1.8 .0325 ! 
15 
2.0 
.0375 
13 2.0 .033 i 
13 
2.3 
0375. 
13 2.0 .035 ' 
12 
2.4 
.038 . 
12 2.2 .035 
11 
2.6 
.040 . 
12 2.8 .0375 
8 
3.3 
.040 
10 2.5 .0375 
8 
3.3 
1 038 
7 
3.7 
Average 
V-2.1 .040 i 
11 
2.3 
.040 
7 
3.6 
p., d. and co. Average 
V=2. 7 
0.0375 
17 
1.6 
.0375 
14 
1.9 
.040 
13 
1.9 
.040 
14 
1.8 
.040 
14 
1.8 
.0425 
11 
' 2.1 
.0425 
10 
2.4 
.0425 
11 
2 1 
.045 
10 
2.2 
.045 
11 
2.0 
.045 
8 
2.8 
.045 
9 
2.5 
Average 
V=2. 1 
B. W. AND CO. 
0.03 
13 
2.6 
.03 
16 
2.2 
.03 
12 
2.7 
.035 
15? 
1.9 
.035 
10 
2.8 
.035 
9 
3.2 
.04.. 
8 
3.1 
.04 
7J 
3.3 
\ vpravp 
V=2. 7 
S. AND D. 
LLOYD BROS. 
0.035. . 
.0375. 
.040. . 
.040. 
.0425. 
.045. 
.045. 
Average. 
15 
1.9 
13 
2.1 
12 
2.1 
10 
2.5 
12 
1.9 
8 
2.9 
9 
2.4 
.... V=2.3 
0.036 
.038 
.038 
.040 
.045 
.045 
.045 
.050 
Average. 
16 
1.8 
10 
2.6 
12 
2.2 
11 
2.3 
10 
2.2 
9 
2.5 
8 
2.8 
7 
2.8 
V=2. 4 
a Calculated as of fluid extract strength. 
Digitalone No. 1. Frog, weight 41 grams, injected with 0.5 c. c. per gram body weight. Five hours later 
heart rate was 16, weak contraction but dilating well. 
Digitalone No. 2.. Frog, weight 40 grams, injected with 0.5 c. c. per gram body weight. Five hours later 
heart still beating, rate 17, contractions weak, but dilating fairly well. 
Digitalone No. 3. Frog, weight 39 grams, injected with 0.5 c. c. per gram body weight. Ventricle stopped 
in fifty-seven minutes; auricles beat two hours longer. 
