Digitalone No. 1. 
August 17, 1908. Frog, 41 grams: 
11.25. Heart rate 27 in twenty seconds. 
11.29. Heart rate 28 in twenty seconds. 
11.30. Injected 2.05 c. c. digitalone No. 1; 0.05 c. c. per gram body weight. 
11.42. Heart rate 18 in twenty seconds. 
11.50. Heart rate 16 in twenty seconds. 
12.01. Heart rate 16 in twenty seconds. 
12.30. Heart rate 17 in twenty seconds. 
2.15. Heart rate 16 in twenty seconds; heart weak. 
4.30. Heart rate 16 in twenty seconds; dilates poorly and very weak contraction. 
Digitalone No. 2. 
August 17, 1908. Frog, 40 grams: 
11.00. ♦ Heart rate 30 in twenty seconds. 
11.05. Heart rate 37 in twenty seconds. 
11.05. Injected 2 c. c. digitalone No. 2. 
11.10. Heart rate 15 (?) in thirty seconds; irregular peristaltic contractions. 
11.18. Heart rate 16 in twenty seconds; normal contractions. 
11.27. Heart rate 16 in twenty seconds. 
11.41. Heart rate 16 in twenty seconds; systole weak. 
11.50. Heart rate 16 in twenty seconds. 
12.30. Heart rate 15 in twenty seconds. 
2.15. Heart rate 17 in twenty seconds; weak. 
4.15. Heart rate 17 in twenty seconds; weak, dilating fairly well. 
Digitalone No. 3. 
August 17, 1908. Frog, 39 grams: 
10.55. Heart rate 26 in twenty seconds. 
11.01. Heart rate 26 in twenty seconds. 
11.02. Injected 1.95 c. c. digitalone No. 3. 
11.06. Heart rate 11 in twenty seconds; systole prolonged. 
11.12. Heart rate 10 in twenty seconds. 
11.16. Heart rate 16 in twenty seconds; dilates little. 
11.40. Heart rate 7 in twenty seconds, little dilatation. 
11.59. Ventricle in systole; auricles, rate 7 in twenty seconds. 
2.30. Auricle stopped; ventricle in systole. 
Specimens Nos. 1 and 2 showed no digitalis action whatever, while No. 3 was quite 
weak. 
To kill mice Digitalone Xo. 1 required from two and one-lialf to five 
times the dose that any other preparation examined required except- 
ing Lloyd’s “Specific Medicine, digitalis.” On guinea pigs 2 milli- 
grams per kilogram body weight caused death, but as pointed out 
earlier it was a question whether this was due to the digitalis per se 
or to the effect of an injection under the skin of 12.5 c. c. of fluid con- 
taining chloretone and possibly digitalis decomposition products. 
The animal became comatose at once, showing no characteristic 
digitalis symptoms. Preparation Xo. 2, tested by the twelve-hour 
method on frogs, did not cause death in doses as high as 0.07 c. c. per 
grain body weight, while the preparations made according to the 
