19 
Table No. 9. — Horse serum, then calcium chloride. 
G. P. No. 
G. P. 
weight. 
First injection. 
Inter- 
val in 
days. 
Second injection. 
Result. 
10010 
Grams. 
600 
0.23 c. c. toxine 7+1/1020 
127 
6. c. c. normal horse serum 
Dead in 15 min- 
10008 
600 
c. c. antitoxic horse se- 
rum (NYBH 310). 
0.23 c. c. toxine 7+1/1040 
127 
intraperitoneally. 5 
minutes later, 0.15 gm. 
5 per cent solution CaCl 2 
subcutaneously. 
6 c. c. normal horse serum 
utes. 
Dead in 20 min- 
10014 
600 
c. c. antitoxic horse se- 
rum (NYBH 310). 
0.23 c. c. toxine 7+1/980 
127 
intraperitoneally. 10 
minutes later, 0.1 gm. 
5 per cent solution of 
CaCl 2 subcutaneously. 
6 c. c. normal horse serum 
utes. 
Dead in 45 min- 
c. c. antitoxic horse se- 
rum (NYBH 310). 
intraperitoneally. 5 
minutes later, symp- 
toms. 10 minutes later, 
0.1 gm. 5 per cent solu- 
tion of CaCl 2 subcuta- 
neously. 
utes. 
Table No. 10. — Controls, magnesium sulphate, toxic dose. 
G. P. No. 
G. P. 
Weight. 
Injection. 
Result. 
Control 
Grams. 
415 
1 gm. 25 per cent solution of MgSC >4 subcutaneously. 
Recovered. 
Do 
535 
0.5 gm. 25 per cent solution of MgSC >4 subcutane- 
Recovered. 
Do 
420 
ously. 
0.6 gm. 25 per cent solution of MgSC >4 subcutane- 
Recovered. 
ously. 55 minutes later, complete narcosis. 0.1 
gm. 5 per cent solution of CaCk subcutaneously. 
W oke up 5 minutes later. 
It is evident from the above tables that the hypnotic substances 
used in these experiments (urethane, paraldehyde, chloral hydrate, 
and magnesium sulphate) had practically no influence upon the fatal 
outcome of anaphylaxis. Of course, on account of the action of the 
hypnotic the evident symptoms of anaphylaxis were masked. 
As shown by the control experiments, the amount of hypnotic used 
was not sufficient in itself to cause the death of the animal. While 
theoretically the use of such substances seemed promising, it seems 
from, our work that they offer little or no practical advantage for 
this purpose. 
