20 
SPECIFICITY. 
In our previous papers we drew attention to the specific nature of 
anaphylaxis, especially when proteins of a sufficiently diverse nature 
are used at the first and second injections. 
Gay and Southard , a in an interesting paper on the relative specifi- 
city of anaphylaxis, report that anaphylaxis in guinea pigs caused 
by horse serum, egg white, or milk, is only relatively specific, and 
that the maximum reaction of the second injection is always ob- 
tained when the substance which has been used to sensitize is injected. 
We now report further work upon the specificity of anaphylaxis 
in the use of horse serum, egg white, and milk, and, as will be seen 
from the tables, our results are not in accord with those of Gay and 
Southard above referred to. 
In the first series (Table Xo. 11) 10 guinea pigs were sensitized 
with 1 c. c. of egg white and thirty days later were divided into three 
lots and tested for susceptibility to horse serum, egg white, and milk. 
The animals that received the horse serum and milk did not show the 
slightest symptoms, while the animals that received the egg white 
(homologous protein) died within a few minutes. 
In the second series (Table Xo. 12), the animals received 0.01 c. c. 
normal horse serum at the first injection; but only those which 
received the homologous protein (serum) at the second injection 
showed any symptoms. 
In the third series (Table Xo. 13) the animals were sensitized with 
1 c. c. of milk, and only those which received the same protein at 
the second injection developed any symptoms. 
Table Xo. 11. — Egg ichite v. horse serum and milh. 
G. P. No. 
1 G. P. 
weight. | 
First injection. 
Inter- 
val in ! 
days. 1 
Second injection. 
Result. 
116 
1 c. c. saturated solution 
egg white subcutane- 
ously. 
30 
6 c. c. normal horse ( F rank ) 
serum intraperitoneally. 
No symptoms. 
117 
do 
30 
do 
Do. 
ns 
do 
30 
do 
Do. 
119 ! 
do 
30 ! 
do 
Do. 
no J 
do 
30 
6 c. c. milk intraperito- 
neally. 
Do. 
m 
do 
30 
do 
Do. 
112 
do 
30 
do 
Do. 
113 
do 
30 
do 
Do. 
114 ! 
do 
30 
do 
Do. 
U5 
30 
6 c. c. egg white intraperi- 
toneally. 
Dead in 15 min- 
utes. 
a Gay, F. G., and Southard. E. E.: The relative specificity of anaphylaxis. Joum. Med. Research, 
vol. 19, No. 1, July, 1908, pp. 5-15. 
