21 
Table No. 12. — Horse serum v. egg white and milk. 
G. P. No. 
G. P. 
weight. 
First injection. 
Inter- 
val in 
days. 
Second injection. 
Result. 
130 
30 
6 c. c. saturated solution 
No symptoms. 
1°9 
(Frank) serum, subcu- 
taneously. 
do 
30 
egg white in salt solu- 
tion, intraperitoneally. 
do 
Do. 
13' 
.do 
30 
^ . ..do 
Do. 
13c 
do 
30 
do 
Do. 
131. 
do 
30 
6 c. c. milk, intraperito- 
neally. 
do 
Do. 
136 
. . .do 
30 
Do. 
137 
.do. . 
30 
. .do. . 
Do. 
138 
do 
30 
do 
Do. 
139 
do 
30 
. .do 
Do. 
134 
do 
30 
6 c.c. normal horse (Frank) 
serum, intraperitoneally. 
Dead in 15 min- 
utes. 
Table No. 13. — Milk v. horse serum and egg white. 
G. P. No. 
G. P. 
weight. 
First injection. 
Inter- 
val in 
days. 
Second injection. 
Result. 
125 
1 c. c. milk, subcutane- 
1 ously. 
do 
30 
6 c.c. normal horse ( Frank) 
serum, intraperitoneally. 
. . .do 
No symptoms. 
Do. 
126 
30 
127 
do 
30 
.do. . . 
Do. 
128 
do 
30 
do 
Do. 
129 
... .do 
30 
.do. . 
Do. 
120 
.do 
30 
6 c. c. saturated solution 
Do. 
121.. 
do 
30 
egg white in salt solu- 
tion, intraperitoneally. 
.. ..do 
Do. 
do 
30 
.. ..do 
Do. 
123 
do 
30 
.do. 
Do. 
124 
do 
30 
6 c. c. milk, intraperito- 
neally. 
Severe symptoms. 
Wells ° reported experiments in which he suggests that probably 
the specificity of horse serum suffers somewhat after long preservation 
in chloroform. 
We sensitized 15 guinea pigs (Table No. 14) with 0.01 c. c. anti- 
toxic horse serum which had been preserved under chloroform about 
two years, and after an interval of twenty-nine days we tested their 
susceptibility with egg white, milk, and normal horse serum. 
The animals that had received, at the second injection, egg white 
and milk showed absolutely no reaction, while those receiving horse 
serum responded in a typical manner. 
“Wells, H. Gideon: Studies on the chemistry of anaphylaxis. Joura. Infec. 
Diseases, vol. 5, No. 4, October 20, 1908, pp. 449-483. 
