37 
Table No. IS —Sensitizing action — Continued. 
HEATED LIQUID MILK. 
G. P. No. 
G. P. 
weight. 
First injection. 
Inter- 
val in 
days. 
Second injection. 
Result. 
1 c. c. whole milk heated 
24 
6 c. c. milk, intraperitone- 
Severe symptoms. 
to 130° C. for 15 min- 
ally. 
utes, subcutaneously. 
1 c. c. whole milk heated 
24 
6 c. c. milk, intraperitone- 
Moderate 
symp- 
to 130° C. for 15 min- 
ally. 
toms. 
utes, subcutaneously. 
1 c. c. whole milk heated 
24 
6 c. c. milk, intraperitone- 
Slight symptoms. 
to 130° C. for 15 min- 
ally. 
utes, subcutaneously. 
1 c. c. whole milk heated 
24 
6 c. c. milk, intraperitone- 
Dead in 
30 min- 
to 130° C. for 15 min- 
ally. 
utes. 
utes, subcutaneously. 
1 c. c. whole milk heated 
24 
6 c. c. milk, intraperitone- 
Moderate 
symp- 
to 130° C. for 15 min- 
ally. 
soms. 
utes, subcutaneously. 
1 c. c. whole milk heated 
24 
6 c. c. milk, intraperitone- 
Slight symptoms. 
to 170° C. for 10 min- 
ally. 
utes. 
1 c. c. whole milk heated 
24 
6 c. c. milk, intraperitone- 
Moderate 
symp- 
to 170° C. for 10 min- 
ally. 
toms. 
utes. 
1 c. c. whole milk heated 
24 
6 c. c. milk, intraperitone- 
Moderate 
symp- 
to 170° C. for 10 min- 
ally. 
toms. 
utes. 
1 c. c. whole milk heated 
24 
6 c. c. milk, intraperitone- 
Moderate 
symp- 
to 170° C. for 10 min- 
ally. 
toms. 
utes. 
1 c. c. whole milk heated 
24 
6 c. c. milk, intraperitone- 
Moderate 
symp- 
to 170° C. for 10 min- 
,ally. 
toms. 
utes. 
In the above experiments the milk was heated to 130° for fifteen 
minutes in an autoclave. The liquid whole milk was brought to a 
temperature of 170° for ten minutes by exposing it in sealed tubes 
in a dry wall oven. 
THE EFFECT OF HEAT UPON SENSITIVE GUINEA PIG SERUM. 
Lewis has reported that the sensitizing action of sensitive guinea 
pig serum is apparently unaffected when heated in liquid blood to 
60° for thirty minutes. In order to determine whether it is modified 
by higher temperatures we collected the blood from five guinea pigs 
which had been sensitized with a toxine-antitoxin mixture. The 
blood was defibrinated, centrifugalized and the serum drawn off. 
Two and a half c. c. of this fresh mixed serum was injected into 
each of two guinea pigs, which were tested forty-eight hours later 
for their susceptibility and found to be sensitive. 
