463 
Table No. 11. — Milk from healthy cow (No. 2) planted 1 \ hours after milking. 
[Controls, same milk boiled— 24-hours old agar culture used.] 
Bacteria 
per loop, 
Bacteria 
per loop, 
Bacteria 
per loop, 
Bacteria 
per loop, 
1 Bacteria per loop, after 
8J hours at 37° C. 
at once 
after in- 
oculation. 
after 2J 
hours at 
37° C. 
after 4i j 
hours at 
37° C. 
after 6J 
hours at 
37° C. 
Moder- 
ately 
shaken. 
Mixed 
with 
pipette. 
B. typhosus in raw milk 
1,880 
1,380 
1,060 
1,480 
1,980 
12, 200 
B. tvpuosus in same milk 
boiled (control) 
2, 120 
4,020 
o b 800, 000 
(“) 
(®) 
(°) 
B. typhosus in raw milk plus 
1 drop typhoid serum 
2, 100 
2,040 
1,920 
2,360 
1,260 
o b 20, 000 
B. typhosus in boiled milk 
plus 1 drop typhoid serum.. 
2,280 
2,360 
7,020 
6,480 
10,860 
a b 60,000 
B. typhosus in bouillon plus 
1,830 
970 
2,920 
9, 180 
11, 160 
a b 100,000 
1 drop typhoid serum 
Original milk 
0 
1 
» 2 
2 
2 
46 
“ Innumerable. & About. 
The typhoid serum used in this experiment was a strongly agglu- 
tinating horse serum (strength over 1 : 10,000). 
It will be seen that boiled milk plus typhoid agglutinin acts much 
the same as the raw milk. The contrast in each case with the con- 
trol (boiled milk) is striking. 
The fact that agglutination plays a prominent role in this phe- 
nomenon is well illustrated in this table by the fact that the bacterial 
clumps may be shaken apart. This was confirmed by the micro- 
scopic examinations of stained smears in each case. 
Table No. 12. — Milk from healthy cow (No. 2). 
[Plantings 1 hour after milking.] 
Bacteria 
per loop 
at once 
after in- 
oculation. 
Bacteria 
per loop 
after 
3 hours at 
37° C. 
Bacteria 
per loop 
after 
5 hours at 
37° C. 
Bacteria per loop after 
7 hours at 37° C. 
Moder- 
ately 
shaken. 
Mixed 
with 
pipette. 
B. typhosus in raw milk 
B. typhosus in boiled milk 
48-hour B. Coli culture in raw milk 
Original milk 
5.400 
6.400 
16,000 
2 
4,680 
5,040 
12,000 
1 
4,720 
a 6100,000 
11,000 
2 
2,250 
° b 200, 000 
9,000 1 
3 
5,400 
® b 200, 000 
a b 40, 000 
112 
a Innumerable. 
b About. 
