Table No. 13. — Milk from healthy cow (No. 2). 
[Plantings 1 hour after milking.] 
Bacteria 
per loop 
at once 
after in- 
oculation. 
Bacteria 
per loop 
after 
3 hours at 
37° C. 
Bacteria | 
per loop 
after 
5 hours at 
37° C. 
Bacteria per loop after 
7 hours at 37° C. 
Moder- | 
ately 
shaken. | 
Mixed 
with 
pipette. 
1 loop faeces and bay emulsion in raw milk. 
720 
660 
480 
420 ! 
4,320 
1 drop faeces and bay emulsion in raw milk. 
4,860 
4,640 
6,000 
3,780 
9, 720 
5 drops faeces and bay emulsion in raw 
milk 
10,500 
7,500 
9,900 
13,000 
20,000 
Table No. 14. — Milk from healthy cow (No. 2) planted 1\ hours after milking. 
[Controls same milk boiled.] 
j Bacteria 
per loop 
at once 
after in- 
ocula- 
tion. 
Bacteria 
per loop 
Bacteria 
per loop 
Bacteria 
per loop 
Bacteria per loop after 
8| hours at 37° C . 
after 21- 
hours at 
37° C. 
after 4| 
hours at 
37° C. 
after 6J 
hours at 
37° C. 
Moder- 
ately 
shaken. 
Mixed 
with 
pipette. 
1 drop cow faeces and hay suspension 
in raw milk 
0 
0 
3 
26 
44 
480 
1 drop cow faeces and hay suspension 
in boiled milk (control) 
11 
43 
18 
80 
1,260 
4 drops cow faeces and hay suspension 
in raw milk 
51 
2 
124 
450 
1,800 
5,400 
4 drops cow faeces and hay suspension 
in boiled milk (control) 
j 
3 
28 
720 
2 
3. 300 
Original milk 
0 
1 
2 
2 
46 
The fact that bacterial clusters may be separated by shaking, etc., 
is still more convincingly demonstrated in many of our other tables 
throughout the remainder of this article. 
THE GERMICIDAL ACTION COMPARED WITH THAT OF BLOOD 
SERUM. 
The following experiment with blood serum was made to compare 
its germicidal property with that of milk. The blood was drawn 
from the jugular vein of a horse, defibrinated and centrifugated 
for fifteen minutes at 1,800 revolutions per minute. In this way a 
fresh serum free of fibrin and cellular elements was quickly obtained. 
Care was exercised throughout the process to avoid contamination. 
The serum was now divided into two portions: (1) Untreated, 
and (2) heated to 60° C. for twenty minutes. This temperature 
was selected as being sufficient to destroy the germicidal property 
without seriously interfering with the agglutinins. 
The heated and the unheated serum was now inoculated with 
twenty-four-hour-old cultures from agar slants. The bacillary emul- 
sion was first drawn in and out of a pipette in order to break up 
clumps. 
i 
I 
