467 
cubic centimeter. The sediment, rich in leucocytes, contained 630 
bacteria per cubic centimeter, and the milk serum 490. 
The milk was revolved in a centrifuge at 1,500 revolutions for 
twenty minutes; the sediment pipetted off was rich in leucocytes. 
The supernatant serum was drawn off and filtered three times 
through asbestos and was therefore free of leucocytes. The milk 
was inoculated with a pure culture of B. lactis aerogenes and B. typho- 
sus. Four test tubes were prepared with each culture, (1) whole 
milk, (2) the sediment rich in leucocytes, (3) the milk serum con- 
taining no leucocytes, and (4) sterilized whole milk used as a control. 
The table gives the number of bacteria per loop on agar, grown at 
37° C. and counted after twenty-four hours. 
These tables eliminate the leucocytes and phagocytosis as a factor 
in the problem we are studying. 
IS THE GERMICIDAL ACTION SPECIFIC? 
Almost all those who have studied this part of the problem con- 
clude that the germicidal action of milk is specific. The following 
experiment confirms these observations. Here we have the same 
milk showing an active power of restraining the growth of typhoid 
and staphylococcus pyogenes aureus, but not paratyphoid A or B. 
Reference to our work upon the influence of heat (vide infra) upon 
this property of milk also indicates its specific nature. 
The table again demonstrates that agglutination plays at least 
some part in the apparent decrease. 
Table No. 16. — Milk from healthy cow {No. 2). 
[Inoculated 1J hours after milking. Controls, same milk heated to boiling. Organisms from 24-hour 
agar culture, using suspension in water of condensation in test tube.] 
Bacteria 
per loop at 
once after 
inocula- 
tion. 
Bacteria per loop after 
10 hours at 37° C. 
Test tube 
moderate- 
ly shaken. 
Vigorous 
agitation 
of culture 
for 5 min- 
utes. 
B. typhosus in raw milk 
1,760 
2,100 
2,740 
2,300 
2,460 
3,200 | 
1,420 
1,830 
| 
7 ; 
1 
1,700 
(a) 
(a) 
(a) 
(a) 
(a) 
900 
6,640 
42 
12, 500 
B. typhosus in control 
B. para typhosug A in raw milk 
(o) 
B. para typhosus A in control 
B. para typhosus B in raw milk 
(a) 
B. para typhosus B in control 
Staph, pyog. aureus in raw milk 
9,100 
Staph, pyog. aureus in control.. . 
Original milk. . 
a I nnum erable, 
