471 
Table No. 10. — Milk from healthy cow (No. 2) inoculated one and one-half hours 
after milking. 
I Controls, same milk heated in Arnold sterilizer to 100° C. for ten minutes. Organisms 
used were from 24-hour agar cultures.] 
Bacteria per 
loop at once. 
Bacteria per 
loop after 8 
hours at 37° 
C., tube 
moderately 
shaken. ' 
Bacteria per 
loop after 8 
hours at 37° 
C., culture 
vigorously 
agitated 5 
minutes and 
mixed with 
a pipette. 
B. lactis aerogenes in raw milk 
B lactis aerogenes control 
1,200 
1,200 
3,440 
2,160 
2,120 
2,660 
4 
4,400 
(°) 
4,107 
(°) 
1,220 
30,000 
6 
14,000 
B. typhosus in raw mi lk 
B typhosus, control 
7, 560 
Staphylococcus pyogenes aureus in raw milk 
Staphylococcus pyogenes aureus, control 
Original milk 
2,070 
a Innumerable. 
The next experiment was designed to show whether the phenome- 
non of germicidal action could not be duplicated by the addition of 
typhoid agglutinating serum to milk that had been boiled. 
Table No. 11. — Milk from healthy cow (No. 2) planted one and one-half hours 
after milking. 
[Controls, same milk boiled — 24-hours old agar culture used.] 
Bacteria 
per loop 
Bacteria 
per loop 
after 21 
hours at 
37° C. 
Bacteria 
per loop 
after 4 1 
hours at 
37° C. 
Bacteria 
per loop ! 
after 61 , 
hours at 
37° C. 
! Bacteria per loop after 
81 hours at 37° C. 
at once 
after in- 
oculation. 
Moder- 
ately 
shaken. 
Mixed 
with 
pipette. 
B. typhosus in raw milk 
1,880 
1,380 
1, 060 
1,480 * 
1,980 
12,200 
B. typhosus in same milk 
boiled (control) 
2,120 
4,020 
a b 800, 000 
( a ) 
(°) 
(a) 
B . t yphosus in raw milk plus 
1 drop typhoid serum 
B. typhosus in boiled milk 
2, 100 
2,040 
1,920 
2,360 ' 
1,260 ! 
a b 20, 000 
plus 1 drop typhoid serum. 
2,280 
2, 360 
7,020 
6,480 
10,860 ! 
a b 60, 000 
B. typhosus in bouillon plus 
1 drop typhoid serum 
1,830 
970 
2,920 
9,180 
11,160 
a b 100, 000 
Original milk 
0 
1 
1 
2 
2 
2 ! 
46 
a Innumerable. 6 About. 
The typhoid serum used in this experiment was a strongly agglu- 
tinating horse serum (strength over 1 : 10,000). 
It will be seen that boiled milk plus typhoid agglutinin acts much 
the same as the raw milk. The contrast in each case with the con- 
trol (boiled milk) is striking. 
