472 
The fact that agglutination plays a prominent role in this phe- 
nomenon is well illustrated in this table by the fact that the bacterial 
clumps may be shaken apart. This was confirmed by the micro- 
scopic examinations of stained smears in each case. 
Table No. 12. — Milk from healthy cotv (No. 2). 
[Plantings one hour after milking.] 
Bacteria 
per loop 
at once 
after in- 
oculation. 
Bacteria 
per loop 
after 
3 hours at 
37° C. 
Bacteria 
per loop 
after 
5 hours at 
37° C. 
Bacteria per loop after 
7 hours at 37° C. 
Moder- 
ately 
shaken. 
Mixed 
with 
pipette. 
B. typhosus in raw milk 
5.400 
6.400 
16,000 
2 
4,680 
5,040 
12,000 
1 
4,720 
a b 100, 000 
11,000 
2 
2,250 
a b 200, 000 
9,000 
3 
5,400 
a b 200, 000 
a b 40, 000 
112 
B. typhosus in boiled milk 
48-hour B. coli culture in raw milk ! 
Original milk 1 
° Innumerable. 6 About. 
Table No. 13. — Milk from healthy cotv (No. 2). 
[Plantings one hour after milking.] 
Bacteria 
per loop 
at once 
after in- 
oculation. 
Bacteria 
per loop 
after 
3 hours at 
37° C. 
Bacteria 
per loop 
after 
5 hours at 
37° C. 
Bacteria pe 
7 hours 
Moder- 
ately 
shaken. 
r loop after 
at 37° C. 
Mixed 
with 
pipette. 
1 loop faeces and hay emulsion in raw milk . 
720 
660 
480 
420 
4,320 
1 drop faeces and hay emulsion in raw milk . 
5 drops faeces and hay emulsion in raw 
4,860 
4, 640 
6,000 
3,780 
9,720 
milk 
10,500 
7,500 
9,900 
13,000 
20,000 
Table No. 14. — Milk from healthy cow (No. 2) planted one and one-half hours 
after milking. 
[Controls same milk boiled.] 
Bacteria 
per loop 
gt once 
Bacteria 
per loop 
after 21 
hours at 
37° C. 
Bacteria 
per loop 
after 41 
hours at 
37° C. 
Bacteria 
per loop 
after 61 
hours at 
37° C. 
Bacteria per loop 
after 81 hours at 
37° C. 
after in- 
ocula- 
tion. 
Moder- 
ately 
shaken. 
! Mixed 
with 
pipette. 
1 drop cow faeces and hay suspension in 
raw milk 
0 
0 
3 
26 
44 
480 
1 drop cow faeces and hay suspension in 
boiled milk (control) 
11 
43 
18 
80 
1,260 
4 drops cow faeces and hay suspension in | 
raw milk 
51 
2 1 
124 
450 
1,800 
5,400 
4 drops cow faeces and hay suspension in j 
boiled milk (control) 
7 
3 ! 
28 
720 I 
3,300 ; 
Original milk j 
-0 
1 
1 
2 
2 1 
2 l 
46 
