476 
Table No. 17. — Milk from healthy cow (No. 2). 
[Inoculated one and one-half hours after milking. Controls, same milk heated to boiling. 
Organisms from 24-hour agar 4 culture, using suspension in water of condensation in test 
tube. ] 
• 
Bacteria 
per loop at ! 
once after 
inocula- 
tion. 
Bacteria per loop after 
10 hours at 37° C. 
Test tube 
mod- 
erately 
shaken. 
Vigorous 
agitation 
of culture 
for 5 min- 
utes. 
■ 
B. typhosus in raw milk I 
B. typhosus in control 
1,760 
2, 100 
2,740 
2,300 
2,460 
3,200 
1,420 
1,830 
1,700 
(«) 
(«) 
(«) 
(«) 
(«) 
900 
6,640 
42 
12, 500 
B. para typhosus A in raw milk 
B. para typhosus A in control 
( a ) 
B. para typhosus B in raw milk 
B. para typhosus B in control 
(«)- 
Staph, pyog. aureus in raw milk 
Staph, pvog. aureus in control 
9, 100 
Original milk 
“ Innumerable. 
THE EFFECT OF DILUTION. 
Whole raw milk from a healthy cow (No. 2) was diluted in the 
proportion of 1 volume of milk to 3 volumes of distilled water. 
Inoculations were made two hours after milking; 24-hour agar cul- 
tures used. 
Table No. 18. 
Bacteria j 
per loop at 
once after 
inocula- 
tion. 
Bacteria per loop after 
10 hours at 37° C. 
Tube 
shaken 
moder- 
ately. 
Culture 
vigorously 
agitated 5 
minutes 
and mixed 
with a 
pipette. 
B. tvphosus in diluted milk (1+3) 
11,000 
35.000 
16.000 ; 
3,000 
5,400 
36,000 
3.000 
1,200 
a 6 160, 000 
4.000 
33 
a 6 360, 000 
25. 000 
15.000 
B. typhosus in same milk undiluted 
B. tvphosus in sterilized milk (control) 
B. laetis aerogenes in diluted milk (1+3) 
67, 000 
92 
B. laetis aerogenes in same milk undiluted 
B. laetis aerogenes in sterilized milk (control) 
a Innumerable. 
b About. 
