514 
diseased before the test was made or were affected by some cause other than 
the tuberculin. 
SUMMARY OF DIRECTIONS FOR MAKING THE TUBERCULIN TEST. 
1. Stable cattle under usual conditions and among usual surround- 
ings, feeding and watering in the customary manner. 
2. Make a physical examination of each animal, and give to each 
one some designation by which the animal will be known throughout 
the test. 
3. Take each animal’s temperature at least three times at two or 
three hour intervals on the day of injection; for instance, at 2, 5, 
and 8 p. m. 
4. At 8 or 10 p. m. inject a dose of tuberculin under the skin in the 
region of the shoulder, using a sterile hypodermic syringe after dis- 
infecting the skin at the seat of injection with a 5 per cent solution 
of carbolic acid or a similar antiseptic solution. 
5. Tuberculin is not always concentrated to the same degree, and 
therefore the dose, which should always appear on the label, varies 
considerably. The dose of imported tuberculin is 0.25 c. c. for an 
adult cow, and before injection is diluted with sterile water to 2 c. c. 
The tuberculin made by the Bureau of Animal Industry is prepared 
so that it will not be necessary to dilute it, and the dose is 2 c. c. for 
an adult animal. Yearlings and 2-year-olds, according to size, should 
receive from 1 to 1J c. c., while bulls and very large animals may 
receive 3 c. c. 
6. At 6 a. m. on the day following the injection of tuberculin com- 
mence taking temperatures, and continue every two or three hours 
until the twentieth hour after injection, at which time if there is no 
tendency for the temperature to rise the test may cease. 
7. A rise of 2° F. or more above the maximum temperature ob- 
served on the previous day, providing the temperature after injection 
exceeds 103.8° F., should be regarded as an indication of tuberculosis. 
Those cases which approximate but do not reach this standard should 
be considered as suspicious, and held for a retest six weeks later, 
giving double the original dose. 
FOOT-AND-MOUTH DISEASE. 
The recent outbreak of foot-and-mouth disease in Michigan, New 
York, Pennsylvania, and Maryland, which started October 16, 1908, 
has been the occasion of many inquiries regarding the effect of this 
disease upon the milk supply and the danger of its transmission to 
man. A brief discussion of these subjects at this time would therefore 
seem appropriate. 
