669 
Experiment No. 3. 
Pint mixed market milk. 
Bottle immersed in water 6 inches, 1 inch out. 
Distinct cream line from standing twenty-four hours. 
Water 
temper- 
ature. 
Milk temperature. 
Time. 
Colonies | 
per loop 
in agar. 
Remarks. 
Top. 
Bottom. 
°C. 
°c. 
°G. 
Minutes. 
23 
20 
13 
4,000 
30 
21 
21 
2\ 
40 
23 
24.5 
3£ 
6,000 
50 
28 
30 
6£ 
60 
35 
36.5 
8£ 
70 
44.5 
44.5 
94 
80 
53 
.58 
13 
81.5 
55 
54.5 
13 
9,000 
85 
60 
58 
13| 
6,000 
Simmering. 
88 
61.5 
59 
14 
Scum. 
89 
63.5 
60.5 
144 
7,500 
90 
65 
62 
141 
92 
67 
64 
154 
600 
95 
70 
68 
16 
1,500 
Boiling. 
96 
75 
73 
18 
900 
77 
76 
19 
(a) 
Scum. 
a Fewer, but numerous. 
Experiment No. 4. 
Pint mixed market milk. 
Bottle immersed in water 44 inches, 24 inches out. 
Water 
temper- 
ature. 
Milk temperature. 
Time. 
Colonies 
per loop 
in agar. 
Remarks. 
Top. 
Bottom. 
°C. 
°C. 
°a 
Minutes. 
22 
21 
15 
4,300 
30 
22 
17 
14 
40 
22.5 
20 
44 
50 
24 
23 
4 

60 
27 
25.5 
5f 
4,500 
70 
31 
32 
64 1 
80 
35 
40.5 
9 

85 
36 
45 
10 
Simmering. 
93 
44 
54 
114 
6,000 
96 
47 
58 
124 
Boiling. 
50 
59 
13 
3,000 
55 
61 
134 
3,200 * 
58 
62 
134 
3,500 
60 
64 
13| 
65 
65 
134 
4,000 
70 
67 
144 
2,000 
73 
1 
68.5 
15 
3,100 
