11 
‘‘Apparently then the bacillus pestis is very sensitive to rapid and 
energetic drying as occurs by higher temperatures and in the desiccator. 
‘ ‘ Slower drying at lower temperatures is much less deadly. In the 
climate of Germany, therefore, a rapid death of the organism in fab- 
rics, etc., is not to be expected, as occurs in the tropics. In the hot 
countries no live bacilli were found in the dust.” 
Eduardo Germano {a) made many observations to determine this 
question. He worked with a culture from the Breslauer Hygienic 
Institute obtained through Krai, and which had been grown through 
many generations in vitro. He used bouillon suspensions of agar cul- 
tures and exposed the infected objects in Petri dishes at room tempera- 
ture, 16° to i^0° C. 
Boom dust mixed loith an emidsion of plague bacilli. 
Time. 
Moist. 
Dry. 
Dried 
over 
sulphuric 
acid. 
— 
— 
— 
Fine sand mixed with emulsion of plague bacilli. 
Time. 
Moist. 
Dry. 
Dried 
over 
sulphuric 
acid. 
Time. 
Moist. 
Dry. 
Dried 
over 
sulphuric 
acid. 
1 day 
+ 
(«)+ 
20 days 
-f 
5 days 
-j- 

25 days., 
4- 


5 days 
+ 


30 days 
+ 


8 days .- 
-j- 


40 days 
4- 


12 days 
-j- 


50 days 


L 16 days 
4 - 


60 days 


^ 
S ; a The dust is not yet dry. 
h The number of colonies is diminished at least to the part of the original. 
Loam {Humus boden) mixed with emidsion of plague bacillus. 
a Die Uebertragung von Infectionskrankheiten durch die Luft. Dr. Eduardo Ger- 
mano. Zeitschrift fur Hygiene, Vol. XXVI, 1897 ; 281. 
