8 
In accordance Tvith this method six estimations were made of the 
formalin solution used in these experiments, with the following results : 
Per cent. 
Test Xo. 1 40. 90 
Test Xo. 2 40. 02 
Test Xo. 3 40. 20 
Test Xo. 4 40. 50 
Test Xo. 5 40. 27 
Test Xo. 6 39. 60 
Average 40. 25 
XoTE. — I desire to acknowledge tke assistance in the latter part of tMs work ren- 
dered by Asst. Surg. L. D. Fricks, United States Marine-Hospital Service. 
POUEIXG THE FORMALIX OX ABSOEBEXT COTTOX. — TI5IE OF EXPOS- 
URE, TWEXTY-FOUE HOURS. 
Ko. 1. 
Four cubic centimeters formalin on cotton in corners of box, cul- 
tures between blankets, twenty-four hours. 
One cubic centimeter formalin poured on pledgets of absorbent cot- * 
ton j)laced in each corner of a box 15 by 15 by 15 inches. Four cubic 
centimeters in all. Moist freshly prepared cultures on slips of filter 
X>aper placed between blankets near the center of the box. Box opened 
after twenty-four hours. 
Layers of blankets . 
Typhoid. 
Anthrax 
spores. 
Diph- 
theria. 
1 
1 

_L 
o 
— 
— 
-r 
3 
— 
— 
— 
5 
— 
-r 
— 

Controls 
— 
— 
— 
Xo. 2. 
One hundred cubic centimeters formalin on cotton in corners of box, 
cultures between blankets, twenty-four hours. 
Twenty-five cubic centimeters of formalin iDoured on pledgets of absorb- 
ent cotton in each corner of a box. One hundred cubic centimeters in 
all. Size of box 2 by 2 by 2 feet. Fresh moist cultures on slips of filter 
paper placed between layers of a blanket at various distances from the 
corners. The blanket was laid at the bottom of the box, smoothed out 
but not pressed down and no weight above it. Exposure, twenty-four 
houi’s. 
Layers of blankets. 
Anthsax 
spores. 
Diph- 
theria. 
1 
4 
6 
— 
I 
Controls 
- 
- 
