has no effect at all upon anthrax spores, though separated by only a 
single layer. 
The next tests were made to determine whether diluting the larger 
amounts of formalin before sprinkling it increased its power of pene- 
tration. 
LARGER AMOUNTS OF FORMALIN DILUTED AND SPRINKLED. — TIME OF 
EXPOSURE, TWENTA^-FOUR HOURS. 
Xo. 28. 
Two cubic centimeters diluted per square foot, sprinkled, blankets, 
twenty-four hours. 
Three cubic centimeters formalin diluted with 5 c. c. of water sj>rinkled 
on a blanket on the bottom of a box 15 by 15 by 15 inches. Fresh moist ; 
cultures on slips of filter paper placed between layers of blankets. Box i 
Dpened after twenty-four hours’ exposure. 
Layers of blankets. 
Typhoid. 
Diph- 
theria. 
1 
9 
3 
4 
5 
Controls 
"T 
Xo. 29. 
Two cuoic centimeters formalin diluted per square foot, sprinkled, 
blankets, twenty-four hours. 
Three cubic centimeters formalin diluted with 10 c. c. of water ; other 
conditions the same as No. 28. 
Layers of blankets. 
Typhoid. 
1 , 
2 y 

3 

4 

5 

Controls 
+ + 
