22 
impregnated with the cultures placed while still moist between the 
layers of blankets. 
Layers of blankets. 
Typhoid. 
Anthrax 
spores. 
Diph- 
theria. 
2 
4 
6 
8 
1 
+ 
10 
' 
Controls 
_L 
-f 
Xo. 33. 
Two and five-tenths cubic centimeters per square foot, sprinkled on 
blankets, twenty-six hours. 
Ten cubic centimeters formalin sprinkled on blanket at bottom of 
a box 2 by 2 by 2 feet. Cultures on slips of filter paper, moist and 
freshly prepared from bouillon. Box opened after twenty-six hours. 
Layers of blankets. 
Typhoid. 
Anthrax 
spores. 
Diph- 
theria. 
1 
2 
(c) 
3 


4 
_ 
-h 
I 

5 

6 
+ 
— 
8 
_L 
Controls 
+ 
-f 
The effects of 2 and 2.7 c. c. formalin per square foot being quite 
constant through 4 layers of blankets, and often penetrating through 5 
and 8 layers for nonspore-bearing forms of organisms, another trial was 
now made to contrast the effect of using the same amount of formalin 
and keeping all the other conditions constant, except pouring the 
formalin on the blanket instead of sprinkling. 
Special attention is called to experiment No. 33. In this test a growth 
was obtained from typhoid through 2 layers of blanket. Such irregu- 
larities occasionally creeping in make an element of uncertainty that 
renders this method of disinfection very questionable. A good method 
of disinfection must have no exceptions. It must invariably kill. 
