23 
ANOTHER TEST BY POURING INSTEAD OF SPRINKLING. — TIME OF 
EXPOSURE, TWENTY-FOUR HOURS. 
No. 34. 
Two and seven-tenths cubic centimeters per square foot, blankets, 
poured, not sprinkled, twenty-four hours. 
Four cubic centimeters formalin, poured, not sprinkled, on a blanket 
laid on the bottom of a box 15 by 15 by 15 inches. 
^ Diphtheria and typhoid cultures from 36- hours- old bouillon growths. 
Anthrax from six days, containing spores. 
i Slips of filter paper impregnated with cultures and jfiaced fresh and 
moist between the layers of sterilized blankets. Box opened after 
twenty- four hours. 
Layers of blankets. 
Typhoid. 
Anthrax 
spores. 
Diph- 
theria. 
o 
4 
— 
“T 
— 
6 
. 
-r 
— 
b 
-f- 
-f 
Controls 
- 
+ 
This experiment again em^ihasizes the importance of sprinkling the 
formalin in small drops ; otherwise the effect is irregular and uncertain. 
CULTURES IN COAT POCKET. 
No. 35. 
Two and seven-tenths per square foot, cultures in coat pocket, twenty- 
four hours. 
Four cubic centimeters formalin sprinkled on a blanket laid on bot- 
tom of a box 15 by 15 by 15 inches. The cultures wrapped in sterile 
paper and placed in the pocket of a coat which was folded and packed 
in the box so that the cultures were in the inside. 
Nature of experiment. 
Typhoid. 
Anthrax 
spores. 
Diph- 
theria. 
Cultures in coat pocket.. 
-r 
_ 
Do ; 
— 
— 
Do 


Controls 
+ 
-t- 
-r 
Experiment No. 35 gives expected result with nousporulating organ- 
ism, but had no effect on anthrax. 
