25 
tures on slips of filter paper, moist and freshly prepared. Opened after 
twenty-fonr hours. 
Layers of blankets. 
Typhoid. 
Anthrax 
spores. 
Diph- 
theria. 
1 
O 
— 
— 
(a) 
3 
J 
— 
— 
5 
6 
— 
+ 
— 

(a) 

8 
-f- 
— 
9 
— 
+ 
— 
10 


Controls 
+ 
-f- 
a Lost. 
It appears that increasing the amount of formalin does not corre- 
spondingly increase the power of penetration. Four cubic centimeters 
per square foot is not more effective than 2.5 c. c. per square foot in 
twenty -four hours’ exposure, although more constant in its results. 
The next series of experiments were made to determine whether 
increasing the time would increase the efficiency by this method. 
From test Xo. 39 all the slips were neutralized with ammonia after 
exposure and before planting in bouillon. 
INCREASING THE TIME OF EXPOSURE FROM TWENTY-FOUR HOURS TO 
FIVE DAYS, ETC. — FORMALIN ON ABSORBENT COTTON IN CORNERS 
OF BOX. 
- . ' Xo. 39. 
Ten cubic centimeters formalin in corners of box. Cultures between 
layei’S of blanket, five days. 
Two and one-half cubic centimeters formalin poured on pledgets of 
absorbent cotton in each corner of a box 15 by 15 by 15 inches. Ten 
cubic centimeters in all. Slips of filter jiaper, moist and freshly iire- 
pared from bouillon cultures, were placed between layers of a dry 
blanket laid on the bottom of the box without pressure. 
On opening, after an exposure of five days, the cotton was found to 
be dry and contained a dejiosit of paraform. 
1 
Layers of blankets. 
Typhoid. 
Anthrax 
spores. 
Diph- 
theria. 
O 
+ 
+ 
+ 
4 
-f 
-b 
-b 
6 
-L 
-i- 
-I- 
8 
-r 
-f- 
-b 
Controls 
-r 
-b 
-b 
