20 
No. 40. 
Fifteen cubic centimeters formalin in corners of box. Cultures 
between layers of blankets, five days. 
Three and one-half cubic centimeters formalin poured on pledgets of 
absorbent cotton in each corner of a box 15 by 15 by 15 inches. Fifteen 
cubic centimeters in all. Slips of filter paper moist and freshly pre- 
pared from bouillon cultures were placed between layers of a dry blanket 
laid on the bottom of the box without pressure. 
On opening, after an exposure of five days, the cotton was found to be 
dry and contained a deposit of paraform. 
Layers of blankets. 
Typhoid. 
Anthrax 
spores. 
Diph- 
theria. 
9 
+ 

4 
+ 
-f 
— 
6 
-f 
+ 
-f 
8 
+ 
+ 
+ 
Controls 
+ 
_L 
No. 41. 
Twenty-five cubic centimeters formalin in corners of box. Cultures 
between layers of blanket, five days. 
Six and one fourth cubic centimeters formalin poured on pledgets of 
absorbent cotton in each corner of a box 15 by 15 by 15 inches. Twenty- 
five cubic centimeters in all. Slips of filter paper moist and freshly pre- 
pared from bouillon culture were placed between layers of a dry blanket 
laid on the bottom of the box without pressure. 
On opening, after an exposure of five days, the cotton was found to 
be dry and contained a deposit of paraform. 
Layers of blankets. 
Typhoid. 
Anthrax 
spores. 
Diph- 
theria. 
9 
-r 
+ 
-b’ 
4 
+ 
+ 
-b 
6 
+ 
-b 
-b 
8 
+ 
+ 
+ 
Controls 
+ 
-f 
+ 
From Nos. 39, 40, and 41, it is plain that even five days’ exposure, 
with the formalin in cotton in the corners of the box has no effect. 
The formaldehyd was deposited as paraform and not enough of the gas 
was liberated to destroy typhoid and diphtheria. This method is 
therefore totally inapplicable. 
Further trials were then conducted by the method of sprinkling, but , 
increasing the time of exposure to two, three, four, and five days. 
