29 
Ko. 47. 
Two and seven-tenths cubic centimeters formalin per square foot. 
Cultures between layers of blanket, ninety hours. 
Four cubic centimeters formalin sprinkled on a blanket at the bottom 
of a box 15 by 15 by 15 inches. 
Slips of filter paper, moist and freshlj- prepared from bouillon cul- 
tures, were placed between layers of dry blankets, ninety hours’ 
exposure. 
Layers of blankets. 
Typhoid. 
Anthrax 
spores. 
Diph- 
theria. 
+ 
4 
— 
— 
6 
— 
+ 
— 
8 
— 
O- 
— 
Controls 
-f 
- 
- 
(a) Light growth. 
From the foregoing four tests, Xos. 44 to 47, it will be seen that 
2.7 c. c. of formalin per square foot will not always penetrate through 
2 layers of blankets and never through more than 4 for anthrax 
spores, and for nonspore-bearing organisms it can not be detrended 
upon through more than 3 layers. 
It is therefore concluded that increasing the time of exposure does 
not increase the power of penetration through blankets. 
However, to make the work complete still larger amounts of formalin 
were used as follows : 
LARGE AMOUNTS OF FORMALIN SPRINKLED ON BLANKETS. — TIME OF 
EXPOSURE, NINETY HOURS. 
No. 48. 
Four cubic centimeters formalin per square foot. Cultures between 
layers of blankets, ninety hours. 
Six cubic centimeters formalin sprinkled on a blanket at the bottom 
of a box 15 by 15 by 15 inches. Slips of filter paper moist and freshl}' 
prepared from bouillon cultures were placed between layers of dry 
blankets, ninety hours’ exposure. 
Layers of blankets. 
Typhoid. 
Anthra.x 
si>orea 
Diph- 
theria. 
•> 
4 
R 
_ 
+ 

8 
— 
-r 
4 - 
Controls 
-H 
-i- 
