80 
Xo. 49. 
Four cubic centimeters formalin per square foot. Cultures between 
layers of blanket, ninety hours. 
Six cubic centimeters formalin sprinkled on a blanket at the bottom 
of a box 15 by 15 by 15 inches. 
Slips of filter paper moist and freshly prepared from bouillon cultures 
were placed between layers of dry blankets, ninety hours' exposure. 
Layers of blankets. 
Typhoid. 
Anthrax 
spores. 
Diph- 
theria. 
O 



4 
— 
— 
— 
6 
— 
— 
8 
— 
— 
— 
10 
— 
— 
— 
Controls 
— 
- 
— 
Xo. 50. 
Five cubic centimeters formalin per square foot. Cultures between 
layers of blanket, ninety hours. 
Eight cubic centimeters formalin sx^rinkled on a blanket at bottom of 
a box 15 by 15 by 15 inches. 
Slips of filter paper moist and freshly prepared from bouillon 
cultures were placed between layers of dry blankets, ninety hours’ 
exposure. 
Layers of blankets. 
Typhoid. 
Anthrax 
spores. 
Diph- 
theria. 
0 
6 
— 
■a)- 
— 
S 
— 
— 
— 
10 
— 
, — 
— 
12 
— 
— 
— 
Controls 
- 
-f- 
- 
a Small growth. 
From Xos. 48, 49 and 50 more constant results are obtained with 4 
and 5 c. c. formalin per square foot with ninety hours’ exposure than 
with 2.5 c. c. per square foot. 
Cultures were always killed through 4 layers of blankets but not 
through 6. 
It is therefore plain that increasing the time of exposure with this 
method over twenty-four hours does not correspondingly increase its 
efficiency. 
