the percentage of sulphur dioxide had been between 9 and 10 per cent. 
The slips of flannel were then removed from their envelopes and planted 
into tubes of ordinary buillou, and these tubes were then placed in the 
incubator at 37° C. and were noted at the end of twenty-four hours. It 
was then seen that all of the organisms except anthrax had been inhib- 
ited. This organism showed a normal growth, apparently not retarded 
in any way by the process to which it had been subjected. But obser- 
vation at the end of another twenty-four hours — that is, forty-eight 
hours in all — showed that the effect on the other organisms had simply 
been inhibitory and not germicidal. There was a growth in all of the 
|j tubes of all of the organisms planted. The question may, therefore, be 
' decided as settled that di'y sulphur dioxide is not an efficient or reliable 
,| germicidal agent for quarantine or epidemic practice. 
' The experiments were then continued as follows : 
! Experiiment No. 1. 
; Bouillon cultures of cholera, colon, diphtheria, typhoid, icteroides, 
I and plague were exposed on slips of filter pajDer inclosed in sealed 
' i envelopes, which envelopes were perforated to allow access of the gas. 
* The cubic capacity of the room used was 500 cubic feet. The percent- 
i age of sulphur dioxide, by actual determination, was 0.6 per cent. The 
! vessel ill which the sulphur was burned was immersed in another vessel 
containing a quantity of water, which was measured before and after 
} j the experiment, and the amount found evaporated was 150 c. c. Time 
of exposure, twenty-four hours. 
Six cultures of each of the above organisms were placed on a shelf 
within the room ; six each were suspended near the ceiling ; two were 
placed on. the floor of the room ; one of each under a heavy carpet upon 
the floor. 
The temiierature at the beginning of the experiment was 31° C., and 
the highest temperature noted during the continuance of the experiment 
was 35° C. 
The results obtained in this experiment were very uniform. No 
single organism survived the i^rocess. The controls in all cases grew. 
Experiment No. 2. • 
The next experiment was identical with the preceding, the same 
i organisms being used, except that the percentage of gas was 0.8 per 
cent and the time of exposure was shortened to twenty hours. The 
results- were the same. All oiganisms subjected to the process were 
killed, while the controls all grew. 
Experiment No. 3. 
Tlie next experiment was made with cultures as before, disposed upon 
the floor of the room, the_shelf, suspended near the ceiling and under 
