14 
PLAGUE CULTURE IX TAP WATER. 
The culture was taken from an agar slant and emulsilied in a little 
bouillon. Four drops of this suspension were added to 15 c. c. of 
sterile tap water and kept under the conditions as stated below. From 
time to time a small quantity of the infected water was drawn off and 
planted for growth, and its virulence tested upon animals. 
Xo. 3 . — Plague Culture ix Tap AVater. 17° to 19 C. 
Time. Result. Remarks. 
j 1 day + 
6 days + 
11 days — Delayed growth. 
17 days — Small amouat of water used. 
20 days — 2 c. c. used. 
2-5 days — Killed mouse in 3 days. 
:>4 days — 
45 days — 
66 days — Killed mouse in 5 days. 
SO days -r- 
96 days -f- Failed to kill mouse. 
116 days 
Material expended, 
Xo. -1 . — Plague Culture ix Tap Water, 22° to 29° C. 
Time. 
Resttlt. 
Remarks. 
1 day 
6 days 
— 
11 days 
Delayed growth. 
Contamination. 
16 days 
— 
20 days 
— 
Killed mouse in 24 h<)urs. 
25 days 
— 
44 days 
— 
•55 days 
— 
71 days 
— 
Killed mouse in 3 'days. 
96 days 
— 
i 
I 
107 days 
— 
1 
1 
Material expended. 
! 
2s o. 5 , — Plague Culture ix* Tap Water, 37° C. 
Time. 
Result. 
Remarks. 
1 day 
-1- 
6 days 
_i_ 
11 days 
— 
17 days 
— 
' 
20 days 
- 
2 c. c. of the water inoculated into ■ 
bouillon. 
24 days 
