15 
This series (Nos. 3, 4, iuid 5) of plaj^ue in water was re])eated, using 
distilled instead of tap 'svater. But, as the suspensions were made in 
bouillon, and four drops of bouillon were added to each test tube con- 
taining 15 c. c. of water, the results can not be taken as showing the 
longevity of the bacillus in distilled water. 
No, 6 . — Plague Culture in Water, 17° to 19° (’. 
Time. 
Result. 
Remarks. 
1 day 
+ 
6 days 
+ 
11 days 
+ 
Delayed growth. 
17 days 
- 
Small amount of water used. 1 
20 days 
+ 
2 c.c. u.sed to inoculate into bouillon. 
25 days 
+ 
Killed mouse in 5 days. ^ 
34 days 
+ 
45 days 
+ 
j 66 days 
4- 
Killed mouse in 3 days. 
80 days 
+ 
96 days 
+ 
Failed to kill mouse. 
116 days 
_ 
Contaminated. 
Material expended. 

i 
No. 7 . — Plague Culture in Water, 22° to 29° C. 
Time. Re.sult. Remarks. 
1 flay , + 
t) days -f 
11 days ..: - Contaminated. 
16 days — Do. 
20 days + 2 e. c. of the water inoculated into 
bouillon. 
25 days -i Killed a mouse in 0 days. 
34 days + 
45 days + Failed to kill mouse. 
71 days + 
96 days — 
107 <lays — 
Material e.x pended. 
No, S. — Plague Culture in Water, :17° ('. 
' I 
Time. Result. Remarks. 
1 day + 
6 days + 
11 days — 
17 days - 
20 days — 
