17 
No. 12, — Bouillon Culture on Filter Paper, 22 ° to 29° C. 
Time. 
Result. 
Remarks. 
3 da vs 
+ 
Delayed growth. Pure culture. 
8 davs 
— 
14 davs 
— 
2o davs 
— 
35 davs 
— 
46 davs 
— 
In the three following’ experiments (Nos. 13, 14, and 15) a pledget 
of cotton soaked wdth sterile water was kept in the petri dish in order 
to see the effect of , a moist atmosphere. Despite this fact the slips 
dried out quickly, but the organism lived a little longer than when 
allowed to diy without the cotton pledget, as in Nos. 9, 10, 11, and 12. 
No. 13. — Bouillon Culture on Filter Paper, 17° to 19° C. 
Time. 
Result. 
Remarks. ; 
3 davs 
+ 
Delayed growth. 
8 davs 
-f 
The slips are quite dry, despite the 
cotton pledget soaked with water. 
14 davs 
-f- 
25 davs 
— 
35 davs 
— 
46 days 
— 
60 davs 
— 
No. 14. — Bouillon Culture on Filter Paper, 22° to 29° C. 
Time. 
Result. 
Remarks. 
2 davs 
Killed mouse iu 18 hours. 
4 davs 
— 
6 davs 
— 
8 davs 
10 davs 
12 days 
14 davs 
— 
No. 15. — Bouillon Culture on Filter Paper, 22° to 29° C. 
Time. 
Re.sult. 
Remarks. 
3 davs 
+ 
8 davs 
+ 
25 davs 
— 
:15 days 
- 
46 ilavs 
- 
60 davs 
_ 
■2 
22188—01 
