18 
plagi:e ox wood. 
Plague could not be kept alive on pine wood, as the following’ tests 
will show. 
Little splinters of white pine were sterilized b}’ heat and soaked in 
a three-day-old bouillon culture of plague, then placed in petri dishes 
and exposed under the following conditions: 
Xo. 16. — Bouillon Culture on Pine Wood, 17° to 19° C. 
Time. Result. 
Remarks. 
2 days — 
4 davs — 
6 davs — 
8 davs — 
10 davs — 
12 davs — 
14 davs — 
Xo. 1/. — Bouillon Culture on Pine Wood, 37° C. 
Time. 
Result. 
Remarks. 
2 davs 
— 
4 davs 
— 
6 davs 
— 
8 davs 
— 
12 davs 
.... — 
14 davs 
— 
Xo. 18. — Bouillon Culture on Pine Wood, 22° to 29° C. 
Time. 
Result. 
Remarks. 
2 davs 

4 davs 
— 
6 davs 
— 
8 davs 
— 
10 davs 
— 
12 davs 
14 davs 
— 
PLAGUE CULTURE OX SAWDUST. 
Sawdust was sterilized and saturated with a four-day-old culture of 
plague, and kept in a test tube stopped with cotton, so as to permit 
drying, under the following conditions: 
One tube in the incubator at 37° C. 
One tube in the cold chamber at 17° to 19° C. 
One tube in a dark room at 22° to 29° C. 
