28 
Xo. 31. — BoriLLox ('rLTi’RE ox SpoxoE, 17° ro 19° (’. 
Time. 
1 
Result. Remarks. 
3 da vs 
+ ; 
.S days 
+ Contaminated with a mould. 
14 davs 
-U 
25 days 
4- 
35 davs 
4- 
4(J davs 
-i- Do. 
W) davs 
- 
70 davs 
- The pieees of sponge are dry. 
80 davs 
Mould eoutami nation. 
97 davs 
Do. 
106 davs 
_ I 
116 days 
— 
Xo. 32 . — Bouillox Celture ox Spox(iE, 22° to 29° C. 
f 
I 
( 
PI.AGUE OX GAKDEX EARTH (ALLOWED TO DRV). 
Giirden oarth was sterilized by heat and well mixed with a vouno- 
])Ouillon culture of plaj^ue. This mixture was kept in a small bottle, 
loo.sely stoppennl with a plug- of cotton, in order to allow the earth 
to dry. 
Three bottles so prepai’ed were exposed under ditferent conditions 
of temperature. 
The organism did not live long under these conditions. 
Xo. 33 . — Bouillox Culture ox Dry (t.\ri)ex Earth, 17° to 19° C . 
Time. Result. Remarks. 
days + 
« days + 
14 days — 
‘2n days — 
35 days — The sponge is still moist. 
4(j days — 
tiO days — 
Time. 
Result. 
Remarks. 
1 dav 
+ 
11 <lavs 
- 
17 davs 
— 
25 davs 
— 
Still moist. 
