24 
No. 34, — Bouillon Culture on Dry Garden Earth, 22° to 29° C. 
Time. 
Result. 
Remarks. 
1 day 
+ 
6 days 
- 
11 days 
- 
17 days 
- 
25 days 
— 
No. 35. — Bouillon Culture on Dry Garden Earth, 37° C. 
Time. 
Result. 
Remarks. 
1 day 
+ 
4 days 
- 
The garden earth is dry. 
6 days 
- 
11 days 
- 
17 days 
- 
25 days 
— 
PLAGUE ON GARDEN EARTH (KEPT MOIST). 
The garden earth was sterilized and mixed with a bouillon culture 
of plague as in the preceding three experiments, the only difference 
being that the bottles were tightl}^ closed with a cork stopper in order 
to keep the earth moist. 
The bacillus lived a much longer time under these conditions, as was 
to be expected. 
The influence of temperature is well shown here, for at 37° C. the 
bacillus died after fourteen days, while at cooler temperature>s it lived 
eighty-four and ninety -three days, all the other conditions being pre- 
cisely similar. 
No. 36. — Plague Culture on Moist Garden Earth, 17° to 19° C. 
Time. 
Result. 
Remarks. 
2 days 
+ 
11 days 
+ 
22 days 
+ 
32 days 
+ 
46 days 
+ 
56 days 
1- 
Failed to kill a mouse. 
83 days 
+ 
Contaminated. 
93 days 
+ 
Do. 
102 days 
Contaminated; still moist. 
113 days 
— 
Contaminated; dry. 
