26 
No. 41. — Bouillon Cultuee on Bone, 37° C. 
Time. 
1 
Result. 
Remarks. 1 
1 
1 dav 
i 
6 days 
— 
The pieces of bone are dry. 
11 (lavs 
— 
Contaminated with a mould. 
17 days 
— 
Do. 
AGAR SUSPE^SIOX OX BOXE DUST. 
The organism died veiy quickly when the infected bone dust was 
permitted to dry, in no case living more than six days. In marked 
contrast, it lived a very long time on the same bone dust when kept 
moist. 
The bone dust came from the cargo of a vessel from Bombav. It 
was sterilized by heat and then saturated with an agar-agar suspension 
of plague in water and thoroughly mixed. The mixture was placed 
in small bottles and in the following three experiments loosely stop- 
pered with cotton so as to permit drying. « 
No. 42. — Bouillon Cultuee on Dey Bone Dust, 22° to 29° C. 
Time. 
Result. Remarks. 
1 day 
— Loosely stoppered so as to dry. . 
4 days 
-r Killed mouse in 3 days. 
6 days 
— 
1 11 days 
— 
17 days 
— 
25 days 
— 1 
1 
No. 43. — Bouillon Cultuee on Dev Bone Dust, 17° to 19° C. 
Time. Result. Remarks. 
1 (lay -r Loosely stoppered so as to dry. 
■1 days -f The bone dust is dry. 
6 days Killed mouse of plague in 18 hours. | 
11 days — 
17 days — 
25 days — 
No. 44. — Bouillon Culture on Dry Bone Du.st, 37° C. 
Time. Result. Remarks. 
1 day -r Loosely stoppered .so as to dry. 
4 days — The bone dust is dry. 
6 days — 
11 days ; — 
17 days ' ^ — Contaminated with a mold. 
25 days ' — 
