28 
No. 47. — Agar Suspension on Moist Bone Dust, 37° C. 
Time. 
Result. 
Remarks. 
1 dav 
-1- 
Kept moist. 
3 da vs 
+ 
5 davs 
-f- 
7 davs 
+ 
14 days 
+ 
21 davs 
“T 
31 davs 
~r 
43 davs 
+ 
Killed a mouse in 8 days. 
52 davs 
+ 
Contaminated with a mold. 
63 davs 
+ 
Failed to kill a mouse. 
74 davs 
+ 
88 davs 
+ 
98 davs 
+ 
The bone dust is still moist. 
109 davs 
+ 
The bone dust is drying out. 
125 days 
“T 
The bone dust is dry. 
131 days 
— 
Contaminated. 
141 davs 
PLAGUE ON CRASH. 
As a rule, plague dies veiy quickly on crash, which would indicate 
that fabrics are not veiy apt to cany the infection. 
Usually it died in a few days, although in one instance it was found 
to be alive on a piece of crash ninet3"-seven da}"s from the time it was 
inoculated. This plainly shows that there are factors in the viabiliU 
of this organism that are but imperfectl}" understood which occasion- 
alh" preserve its vitalit^L 
The small squares of crash were sterilized and saturated with a three- 
day broth culture of plague and allowed to diy in Petri dishes. 
No. 48. — Bouillon Culture on Crash, 17° to 19° C. 
Time. 
Result. 
Remarks. 
2 davs 

4 days 
- 
6 days 
— 
8 days 
- 
10 davs 
— 
12 days 
— 
14 days 
— 
No. 49. — Bouillon Culture on Crash, 22° to 29° C. 
Time. 
Result. 
Remarks. 
2 davs 
4 davs 
6 davs 
8 davs 
10 davs 
12 davs 
14 davs 
- 
