30 
No. 54 . — Bouillon Cultuke on Cka}?h, 17° to 19° C. 
Time. 
Result. Remarks. 
3 davs 
— Permitted to drv in Petri dish. 
8 davs 
_L 
14 davs 
4- 
25 davs 
-r Mould contamination. 
35 davs 
4- * Failed to kill mouse. ' 
46 davs 
4- ■ 
60 davs 
4- 
70 davs 
1 
. . . - , T 
80 davs 
4- Contaminated. 
97 davs 
"T 
106 davs 
.... - Do. 
116 davs 
.... - Do. 
i 
4 
■ 
■ 
PLAGUE OX CAEPET AXD PLUSH. ! 
j 
Plague could not be made to live on carpet and plush. Many sam- j 
pies of carpet and plush were sterilized and inoculated with plague, but | 
from none was the organism recovered. It is possible that the dye- | 
stutf has something to do with this result, for all the samples used had 
soluble dyes. Plague could be grown in the solution of the dyes, so 
that the dyes did not possess the power of preventing growth. It is 
not evident why plague should not live in carpet and plush as well as 
it does on crash and paper, and the results emphasize the fact that 
there are factors that influence the viability of the germ that are not 
well understood. 
PLAGUE IX MILK. 
According to all reported works upon the subject, and the three 
tests that follow, milk is a good medium for plague. 
Three test tubes of milk were sterilized and inoculated with a two- 
day-old agar culture of plague, one loop of culture used for each tube. 
The three tubes were incubated at 37^ C. for one day, then placed 
under difi'erent conditions: 
One left in the incubator at 37^ C. . i 
One left in the cold chamber at 17^ to 19^ C. 
One left in a dark room at '22^ to 29^ C. 
From time to time small quantities of the milk were transferred to 
bouillon for growth. The milk was contaminated, which contamination 
made the identification of the plague bacillus more difficult and doubt- 
less influenced its longevity. 
