31 
No. 5o.— M ilk, in the Incubator at 37° C. 
Time. 
Re.snlt. 
Remarks. 
9 dav.s 
(?) 
Contaminated. 
12 dll vs 
(?) 
Do. 
22 da vs 
+ 
Killed mouse of plague in 3 days. 
33 da vs 
+ 
49 da vs 
+ 
.58 days 
— 
Contaminated. 
68 davs 
Do. 
No. 56. — Milk, in the Cold Chamber at 17° to 19° C. 
1 Time. 
1 
Result. 
Remarks. 
1 
1 8 davs 
-1- 
Contaminated. 
22 davs 
+ 
32 davs 
+ 
Failed to kill mouse. 
49 davs 
— 
Contaminated. 
58 davs 
— 
Do. 
1 68 davs 
— 
Do. 
No. 57. — Plague 
IN 31ilk, 22° TO 29° C. 
Time. 
Re.sult. 
Remarks. 
9 davs 
+ 
22 davs 
+ 
49 davs 1 
+ 
58 ilavs 
— 
No growth of any kind. 
68 davs '. 
+ 
Pure and typical plague. 
79 davs 
No growth from larger amount of 
milk inoculated. 
99 davs 
— 
I*LA(JUK (’ULTUKP: in cheese. 
The orj^anisni lived seventeen days in cheese. 
American cheese vas melted in a water hath, ])oured into test tubes, 
and sterilized by the fractional method at low temperatures. 
Four cubic centimeters of a three-day-old growth of plague in bouil- 
lon was thoroughly mixed up with 15 c. c. of cheese in each of three 
tubes. These tul)es were then exi)osed under ditlerent conditions of 
temperature. 
(1) In the incubator at 87° C. 
(^2) In the cold chamber at 17 to C. 
(3) In a dark room at 22° to 2b° C. 
