35 
i These results correspond with those of Hankin, who, following dif- 
I ferent methods, found that plague lived but a short time (few days) on 
grain. 
’ PLAGUE BLOOD AND TISSUES. 
< 
I 
Plague may live a considerable time in the tissues and organs of 
I animals dead of that disease, when these substances are preserved under 
special conditions. It is probable that the saprophrytes, which soon 
invade the organism of animals buried or disposed of in the usual way, 
soon overpower and determine the death of the plague organism. In 
this work, when the plague bacillus could be protected from such 
influences, it was found to remain alive and A'irulent for sixty-five days 
I in a piece of dried spleen. ^ 
I The spleen of a rabbit dead of experimental plague was placed with 
I aseptic precautions in a petri dish and kept in the cold chamber at 17° 
i to 19° C. The spleen soon dried out. Pieces were taken from time 
to time and planted in bouillon. 
No. 69. — Spleen of Plague Rabbit. 
Time. Result. 
Remarks. 
6 days -1- 
18 days + 
The spleen is yery dry. 
20 days + 
34 days + 
43 days -i- 
65 days + 
Killed mouse in G days. 
Material expended 
1 
No. 70. — Spleen of Plague Rabbit. 
The spleen of a rabbit dead of experimental jilague was placed in a 
Petri dish, but first the surface was sterilized by moistening and burn 
ing with alcohol, and kept in a dark room at ^'2° to 29° C. 
Time. 
Result. 
Remarks. 
1 day 
+ 
13 days 
27 days 
