86 
Xo. 71 . — Spleen of Plague Rabbit. 
The entire spleen of a rabbit dead of experimental plague was kept 
in a Petri dish in the incubator at 37° C. The spleen soon dried out 
and became hard and friable. Small pieces were taken from time to 
time and planted into bouillon. 
Time. 
Result. 
Remarks. 
1 day 
-U 
12 days 
+ 
Killed mouse of plague in 18 hours. 
26 days 
+ 
35 days 
+ 
Contaminated. 
45 days 
+ 
Failed to kill mou.se. 
Material expended. 
PLAGUE BLOOD ON VARIOUS OBJECTS. 
Plague-infected blood from animals dead of septicaemic plague was 
inoculated upon various objects, such as paper, wood, crash, sponge, 
etc. , and kept under various conditions of temperature. 
It was found that such thin, diy layers of blood will not preserve 
the bacillus very long— at most nine days. 
No. 72 . — Plague Blood on Filter Paper. 
The heart’s blood of a rabbit dead of experimental plague was 
inoculated on little slips of white filter paper. These slips were 
placed in a Petri dish and allowed to dr}^ out in a dark room at 22° 
to 29° C. 
Time. 
Result. 
Remarks. 
2 days 
+ 
Killed rabbit over night by intrave- 
nous inoculation. 
4 days 
+ 
6 days 
— 
13 days 
— 
25 days 
— 
No. 73 . — Plague Blood on Crash. 
The heart’s blood of a rabbit dead of experimental plague was 
inoculated on little squares of sterile fabric (crash) and then allowed 
to diy out in a Petri dish exposed in a dark room at 22° to 29° C. 
Time. 
Result. 
Remarks. 
2 days 
+ 
4 days 
6 days 
— 
13 days 
— 
25 davs 
— 
~ 
