13 
The bacteria were exposed as follows : 
■ 
Garden 
earth. 
Subtilis 
spores. 
Anthrax 
spores. 
Staphy- 
lococcus. 
Pyocya- 
neus. 
Icter- 
oides. 
In envelopes, not sealed 
+ 
+ 
-f 
In Petri dishes, open 
In paper boxes 
-f 
The mosquitoes were exposed as follows ; 
In a battery jar — 
1. Covered with mosquito netting only 
2. One-third full of crumpled toweling and tissue paper loosely 
tucked in to afford a hiding place 
3. Two-thirds full of paper and toweling to afford a hiding place... 
In pill boxes — 
1. Between sheets of a folded newspaper 
2. Under 1 sheet of newspaper 
3. Under 1 layer of dry toweling 
4. Under 2 layers of dry toweling 
5. Wrapped in 1 layer of damp toweling 
6. Wrapped in 2 layers of damp toweling 
7. Wrapped tightly in small damp towel 
8. Wrapped twice in a damp towel and that covered with a dry 
towel 
9. Stuck to wall with a damp towel hung directly over the box 
10. Same with a dry towel hung over it, 1 inch from box 
11. Same with 2 dry towels hung over the box 
12. In outside overcoat pocket, 8 inches deep 
13. In inside pocket of overcoat 
* 
14. On floor, under 2 crumpled towels, piled in a heap 
15. On floor under a pile of towels 
Dead. Alive. 
15 0 
1 11 
0 12 
0 2 
8 0 
6 0 
1 2 
7 0 
6 1 
0 4 
0 4 
8 1 
14 0 
8 0 
0 7 
0 5 
0 4 
0 2 
The mosquitoes and the cultures were not exposed under precisely 
similar conditions, so that an exact comparison can not be drawn between 
the insecticidal and germicidal power of th« gas in this test. 
Here it is again evident from the tests made with the battery jars that 
the mosquitoes have the ability to protect themselves by seeking hiding 
places in the folds of the towel and tissue paper. 
In this test the high percentage of the gas had sufficient germicidal 
power to kill all the non-spore-bearing organisms and many of the spores 
during the short exposure. • 
No. 10. 
This test was made with the autoclave in the same room of 2,000 cubic 
feet capacity described above. The gas was allowed to pass into the 
room for fifteen minutes under a pressure of between 45 and 50 pounds 
to the square inch. As before, 2,000 c. c. (about 65 ounces) of the glyco- 
chloro-forinol solution were used and 1,200 c. c. passed over, leaving a 
residue of 800 c. c. in the retort. 
