LABORATORY COURSE IN PATHOLOGY AND BACTERIOLOGY. 
HYGIENIC LABORATORY, 
United States Public Health and Marine-Hospital Service, Washington, D. C. 
[By Milton J. Rosenau, Passed Assistant Surgeon, United States Public Health and Marine-Hospital Service, 
and Director of the Hygienic Laboratory.] 
PREPARATION OF MEDIA— 
1 . Bouillon : 
а. Acid. 
б. Neutral, 
c. Alkaline. 
2. Sugar free bouillon (colon fermentation). 
3. Glucose bouillon. 
4. Lactose bouillon. 
5. Dunham’s solution. 
6. Gelatin. 
7. Agar-agar: 
a. Glycerin agar. 
h. Litmus agar. 
8. Potato. ' 
9. Blood-serum : 
a. Fluid. 
h. Coagulated. 
e. Loffler’s blood-serum mixture. 
10. Milk. 
11. Litmus milk. 
12. Aqueous humor of ox eyes. 
13. Ascitic fluid. 
14. Urine. 
