POINTS TO BE OBSERVED IN DESCRIBING AN ORGANISM— 
1. Its source. 
2. Hanging drop. Form, size, grouping and motility. 
3. Spores. 
4. Staining peculiarities. 
5. Flagella. 
6. Appearance of colonies on gelatine, agar, blood-serum, potato, etc. 
7. Relation to oxygen. 
8. Growth on gelatin. 
y. Growth on agar. 
10. Growth on blood-serum. 
11. Growth on potato. 
12. Growth on fluid media: 
a. Bouillon — 
1. Acid. 
2. Neutral. 
3. Alkaline. 
b. Dunham’s solution — indol. 
13. Milk and litmus milk. 
14. Reaction of media best suited to growth. 
15. Changes in reaction of media. 
16. Gas production : 
a. Glucose bouillon. 
b. Lactose bouillon. 
c. Glucose agar. 
d. Glucose gelatin. 
17. Temperature best suited to development. 
18. Pathogenicity. 
1 9. Reaction to sera of immune animals. 
20. Toxins. 
