10 
MAKING PLATES. 
1. Kocli’s method — glass plates. 
2. Esmarch’s roll tubes. 
3. Slants of agar and gelatin in test tubes. 
4. Petri dishes — 
Streaking on surface with platinum wire. 
Daubing with swabs on surface. 
Mixing while fluid. 
Obtaixixg puee Cultuees — 
1. By plate method on solid media. 
2. By dilution in liquid media. 
3. By temperature. 
4. Inoculation into animals. 
5. Negative plates. 
6. Special methods. 
Obseeve Geowth and Multiplication of Bacteeia in Cell Slide — Agae 
Method : 
1. Division of bacilli. 
2. Division of cocci. 
3. Sporulation. 
Study of Beownian Movement with : 
India ink and other substances in hanging drop. 
