15 
PREPARATION AND MOUNTING OF HISTOLOGIC SPECIMENS— 
1. Fixation ; 
a. Acetic-bichlorid of mercury. 
b. Mullers fluid. 
c. Chrom-acetic acid. 
d. Flemming’s solution. 
e. Formaldehyd. 
/. Absolute alcohol. 
2. Dehydration : 
a. Alcohol 70 per cent (iodine alcohol if Hg Clj). 
b. Alcohol 85 per cent. 
c. Alcohol 95 per cent. 
d. Absolute alcohol, twice. 
3. Clearing ; 
a. Xylol. 
b. Chloroform. 
c. Turpentine. 
d. Oil cloves. 
e. Oil bergamot. 
/. Oil origanum. 
4. Infiltration and imbedding : 
a. Paraffin. 
b. Celloidin. 
5. Sectioning : 
a. Single sections. 
b. Serial sections. 
6. Attaching section to slide : 
a. Alcohol 85 per cent. 
b. W ater. 
c. Myer’s albumen. 
d. Gum Arabic. 
e. Collodium and clove oil. 
7. Removal of imbedding material : 
a. Xylol to remove paraffin. 
b. Alcohol and water. 
8. Staining : 
a. Nuclear staining. 
b. Protoplasmic. 
c Regressive. 
d. Gram’s. 
e. Bacterial. 
/. Special. 
9. Dehydration in absolute alcohol, aniline oil : 
10. Clearing : 
a. Xylol. 
b. Turpentine. 
c. Oil origanum. 
d. Oil bergamot. 
e. Oil cloves. 
/. Chloroform. 
11. Mounting in xylol balsam : 
