37 
BLOOD. 
Technique of:' 
1. Collecting blood for microscopic examination. 
2. Collecting blood for bacteriologic examination — 
a. Small amount. 
h. Large quantities. 
Of man. 
Of other mammals. 
Of birds. 
Of reptiles. 
3. Observe circulation of blood under microscope. 
Mesentery of frog. 
4. Estimate alkalinity. 
5. Estimate specific gravity of blood with mixture of chloroform and benzol. 
6. Examine fresh blood in hanging drop. 
7. Examine fresh blood betvs^een slide and cover slip. 
8. Study of blood films : 
a. Method with cover glass. 
h. Method with special rod. 
c. Method with cigarette paper. 
9. Fixation of blood films : 
a. Heat, 150° C. for about five minutes. 
h. Alcohol and ether. 
c. Formalin. 
d. Formaldehyd. 
e. Acetic acid bichlorid. 
10. Staining. 
a. Plhrlich-Biondi-tri-acid stain. 
h. Jenner’s stain. 
c. Romanowsky’s stain. 
d. Goldhorn’s fiuid. 
e. Eosin and methylen blue. ■ 
/. Thionin and eosin. 
11. Counting red corpuscles. 
Thoma-Zeiss hemocytometer. 
12. Estimating the number of leucocytes : 
a. With the Thoma-Zeiss hemocytometer. 
h. Comparative count of — 
a. Small lymphocytes. 
b. Large lymphocytes and transitional forms. 
c. Polymorphonuclear neutrophiles. 
d. Eosinophiles. 
e. Mast cells. 
13. Estimating the amount of hemoglobin : 
With— 
a. Dare’s hemoglobinometer. 
b. Oliver’s hemoglobinometer. 
c. Gower’s hemoglobinometer. 
